{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:14:21Z","timestamp":1792516461520,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T00:00:00Z","timestamp":1683072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101121"],"award-info":[{"award-number":["82101121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An elevation of pathologic intraocular pressure (IOP) is the greatest risk factor for glaucoma. CD154 has been reported to bind to CD40 expressed by orbital fibroblasts and be involved in immune and inflammatory responses. However, the function and mechanism of CD154 in ocular hypertensive glaucoma (OHG) are not fully understood. We isolated and characterized M\u00fcller cells and subsequently examined the effect of CD154 on ATP release from those cells. After being cocultured with CD154\u2010pretreated M\u00fcller cells, retinal ganglion cells (RGCs) were treated with P2X7 siRNAs or a P2X7 inhibitor. Furthermore, mouse models of glaucoma (GC) were injected with P2X7 shRNA. p21, p53, and P2X7 expression were examined, and cellular senescence and apoptosis were detected by \u03b2\u2010Gal and TUNEL staining, retinal pathology was examined by H&amp;E staining, and CD154 and \u03b2\u2010Gal expression were detected by ELISA. CD154 induced ATP release from M\u00fcller cells and accelerated the senescence and apoptosis of RGCs that had been cocultured with M\u00fcller cells. We also found that treatment with P2X7 could attenuate the senescence and apoptosis of RGCs mediated by M\u00fcller cells pretreated with CD154. In vivo studies in GC model mice verified that P2X7 silencing attenuated pathological damage and prevented the senescence and apoptosis of retinal tissue. The study demonstrates how CD154 accelerates the aging and apoptosis of RGCs by co\u2010cultivating M\u00fcller cells pretreated with CD154 in OHG. The research implies that CD154 has the potential to become a new therapeutic target for ocular hypertension glaucoma, providing a new research direction for its treatment.<\/jats:p>","DOI":"10.1002\/cbin.12030","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T09:34:04Z","timestamp":1683106444000},"page":"1441-1452","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Insights into CD154\u2010mediated pathways in ocular hypertensive glaucoma: The role of M\u00fcller cells and P2X7 in retinal neuroprotection and therapeutic potential"],"prefix":"10.1002","volume":"47","author":[{"given":"Huiling","family":"Hu","sequence":"first","affiliation":[{"name":"Shenzhen Eye Hospital, Jinan University Shenzhen Eye Institute  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Eye 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