{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:56:07Z","timestamp":1791910567940,"version":"4.3.4"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600234"],"award-info":[{"award-number":["81600234"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010846","name":"Health and Family Planning Commission of Hubei Province","doi-asserted-by":"publisher","award":["WJ2021M064"],"award-info":[{"award-number":["WJ2021M064"]}],"id":[{"id":"10.13039\/501100010846","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Alleviating myocardial ischemia\u2010reperfusion injury (MIRI) plays a critical role in the prognosis and improvement of cardiac function following acute myocardial infarction. Pyroptosis is a newly identified form of cell death that has been implicated in the regulation of MIRI. In our study, H9c2 cells and SD rats were transfected using a recombinant adenovirus vector carrying cFLIP<jats:sub>L<\/jats:sub>, and the transfection was conducted for 3 days. Subsequently, H9c2 cells were subjected to 4\u2009h of hypoxia followed by 12\u2009h of reoxygenation to simulate an in vitro ischemia\u2010reperfusion model. SD rats underwent 30\u2009min of ischemia followed by 2\u2009h of reperfusion to establish an MIRI model. Our findings revealed a notable decrease in cFLIP<jats:sub>L<\/jats:sub> expression in response to ischemia\/reperfusion (I\/R) and hypoxia\/reoxygenation (H\/R) injuries. Overexpression of cFLIP<jats:sub>L<\/jats:sub> can inhibit pyroptosis, reducing myocardial infarction area in vivo, and enhancing H9c2 cell viability in vitro. I\/R and H\/R injuries induced the upregulation of ASC, cleaved Caspase 1, NLRP3, GSDMD\u2010N, IL\u20101\u03b2, and IL\u201018 proteins, promoting cell apoptosis. Our research indicates that cFLIP<jats:sub>L<\/jats:sub> may suppress pyroptosis by strategically binding with Caspase 1, inhibiting the release of inflammatory cytokines and preventing cell membrane rupture. Therefore, cFLIP<jats:sub>L<\/jats:sub> could potentially serve as a promising target for alleviating MIRI by suppressing the pyroptotic pathway.<\/jats:p>","DOI":"10.1002\/cbin.12091","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T11:10:46Z","timestamp":1695726646000},"page":"60-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["cFLIP<sub>L<\/sub> alleviates myocardial ischemia\u2010reperfusion injury by regulating pyroptosis"],"prefix":"10.1002","volume":"48","author":[{"given":"Dong","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5948-5987","authenticated-orcid":false,"given":"Hui","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzhao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"QingZhuo","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YanFang","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Disease China Three Gorges University Yichang China"},{"name":"Department of Cardiology Yichang Central People's Hospital Yichang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.718122"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-00630-3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.02.006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000985"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-032521-102529"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13186"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2022.11.001"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2022.11.016"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.114537"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.114701"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14155"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.02.023"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2021.12.002"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2022.13521"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101534"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.647116"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16043-9"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14020373"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2023.2166937"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10557-021-07280-1"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.numecd.2021.02.026"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.06.007"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04574-6"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.950798"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay3878"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2015_448"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2cs00757f"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.71086"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2014.03.005"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110111"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10070655"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-022-01022-y"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-022-01365-6"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.002"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-022-00462-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112432"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24076126"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1007341"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114545"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c22114"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.60292"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.01364-21"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.241809"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2023.101884"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01081-y"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2022.08.007"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154681"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00507-5"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105748"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1109938"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5992436"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.175621"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17523"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116304"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-023-01407-7"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-022-09856-4"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.12091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T09:49:56Z","timestamp":1703670596000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.12091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,26]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/cbin.12091"],"URL":"https:\/\/doi.org\/10.1002\/cbin.12091","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,26]]},"assertion":[{"value":"2023-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}