{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:40:05Z","timestamp":1747147205972,"version":"3.40.5"},"reference-count":70,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T00:00:00Z","timestamp":1697587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Geranylgeranyltransferase type I (GGTase\u2010I) significantly affects Rho proteins, such that the malignant progression of several cancers may be induced. Nevertheless, the effect and underlying mechanism of GGTase\u2010I in the malignant progression of salivary adenoid cystic carcinoma (SACC) remain unclear. This study primarily aimed to investigate the role and mechanism of GGTase\u2010I in mediating the malignant progression of SACC. The level of GGTase\u2010I gene in cells was stably knocked down by short hairpin RNA\u2010EGFP\u2010lentivirus. The effects of GGTase\u2010I silencing on the migration, invasion, and spread of cells were examined, the messenger RNA levels of GGTase\u2010I and RhoA genes of SACC cells after GGTase\u2010I knockdown were determined, and the protein levels of RhoA and RhoA membrane of SACC cells were analyzed. Moreover, the potential underlying mechanism of silencing GGTase\u2010I on the above\u2010mentioned aspects in SACC cells was assessed by examining the protein expression of ROCK1, MLC, p\u2010MLC, E\u2010cadherin, Vimentin, MMP2, and MMP9. Furthermore, the underlying mechanism of SACC cells proliferation was investigated through the analysis of the expression of cyclinD1, MYC, E2F1, and p21<jats:sup>CIP1\/WAF1<\/jats:sup>. Besides, the change of RhoA level in SACC tissues compared with normal paracancer tissues was demonstrated through quantitative reverse\u2010transcription polymerase chain reaction and western blot experiments. Next, the effect after GGTase\u2010I silencing was assessed through the subcutaneous tumorigenicity assay. As indicated by the result of this study, the silencing of GGTase\u2010I significantly reduced the malignant progression of tumors in vivo while decreasing the migration, invasion, and proliferation of SACC cells and RhoA membrane, Vimentin, ROCK1, p\u2010MLC, MMP2, MMP9, MYC, E2F1, and CyclinD1 expression. However, the protein expression of E\u2010cadherin and p21<jats:sup>CIP1\/WAF1<\/jats:sup> was notably upregulated. Subsequently, no significant transform of RhoA and MLC proteins was identified. Furthermore, RhoA expression in SACC tissues was significantly higher than that in paracancerous tissues. As revealed by the results of this study, GGTase\u2010I shows a correlation with the proliferation of SACC through the regulation of cell cycle and may take on vital significance in the migration and invasion of SACC by regulating RhoA\/ROCK1\/MLC signaling pathway. GGTase\u2010I is expected to serve as a novel exploration site of SACC.<\/jats:p>","DOI":"10.1002\/cbin.12096","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T05:32:48Z","timestamp":1697693568000},"page":"174-189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Silencing geranylgeranyltransferase I inhibits the migration and invasion of salivary adenoid cystic carcinoma through RhoA\/ROCK1\/MLC signaling and suppresses proliferation through cell cycle regulation"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3362-5616","authenticated-orcid":false,"given":"Zengpeng","family":"Chi","sequence":"first","affiliation":[{"name":"Department of Stomatology Qingdao West Coast New District Central Hospital Qingdao China"},{"name":"Department of Stomatology, Qingdao Hospital University of Health and Rehabilitation Sciences (Qingdao Municipal 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