{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T07:17:32Z","timestamp":1775546252387,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:00:00Z","timestamp":1704758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The rapid rise in propofol dependency and abuse has highlighted limited resources for addressing substance abuse\u2010related cognitive impairment, prompting the development of novel therapies. Dysregulated autophagy flow accelerates neuronal cell death, and interventions countering this dysregulation offer an appealing strategy for neuronal protection. Curcumin, a potent natural polyphenol derived from turmeric rhizomes, is renowned for its robust antineurotoxic properties and enhanced cognitive function. Utilizing CCK\u20108 and Ki67 fluorescent staining, our study revealed that curcumin treatment increased cell viability and proliferative potential in MN9D cells exposed to propofol\u2010induced neurotoxicity. Furthermore, enzyme\u2010linked immunosorbent assay and western blot analysis demonstrated the partial restoration of dopamine synthesis, secretion levels, and TH expression in damaged MN9D cells treated with curcumin. Scanning electrode microscope images displayed reduced autolysosomes and phagosomes in curcumin\u2010treated cells compared to the propofol group. Immunoblotting revealed that curcumin mitigated the degradation of LC3I to LC3II and p62 induced by propofol stimulation, with green fluorescence expression of LC3 postcurcumin treatment resembling that following autophagy inhibitor HCQ treatment, indicating that modulating autophagy flow can alleviate propofol's toxic effects. Moreover, curcumin treatment upregulated the Akt\/mTOR\/p70S6K signaling pathway, suggesting that curcumin potentially curtails autophagy dysregulation in nerve cells by activating Akt\/mTOR\/p70S6K. In conclusion, our findings suggest that curcumin can ameliorate propofol abuse\u2010induced neurotoxicity, partially through autophagy regulation and Akt\/mTOR\/p70S6K signaling activation.<\/jats:p>","DOI":"10.1002\/cbin.12117","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T06:44:30Z","timestamp":1704869070000},"page":"461-472","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Curcumin inhibits propofol\u2010induced autophagy of MN9D cells via Akt\/mTOR\/p70S6K signaling pathway"],"prefix":"10.1002","volume":"48","author":[{"given":"Hongxia","family":"He","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Affiliated Hospital of North Sichuan Medical College Nanchong Sichuan China"},{"name":"Development and Regeneration Key Lab of Sichuan Province, Department of Histology and Embryology, Department of Pathology Chengdu Medical College Chengdu Sichuan China"},{"name":"Mianyang Key Laboratory of Anesthesia and Neuro\u2010regulation, Department of Anesthesiology Mianyang Central Hospital Mianyang Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuping","family":"Han","sequence":"additional","affiliation":[{"name":"Development and Regeneration Key Lab of Sichuan Province, Department of Histology and Embryology, Department of Pathology Chengdu Medical College Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuyan","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Affiliated Hospital of North Sichuan Medical College Nanchong Sichuan China"},{"name":"Development and Regeneration Key Lab of Sichuan Province, Department of Histology and Embryology, Department of Pathology Chengdu Medical College Chengdu Sichuan China"},{"name":"Mianyang Key Laboratory of Anesthesia and Neuro\u2010regulation, Department of Anesthesiology Mianyang 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