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It has been revealed that the iron metabolism of the mother is crucial in fertility. Iron metabolism is strictly controlled by hepcidin, the principal iron regulatory protein. The inflammatory cytokines can modulate hepcidin synthesis and, therefore, the iron metabolism of the endometrium. It was proven recently that FKN, a unique chemokine, is implicated in maternal\u2013fetal communication and may contribute to endometrial receptivity and implantation. In the present study, we investigated the effect of activated THP\u20101 macrophages and FKN on the iron metabolism of the HEC\u20101A endometrial cells. We established a noncontact coculture with or without recombinant human FKN supplementation to study the impact of the macrophage\u2010derived factors and FKN on the regulation of hepcidin synthesis and iron release and storage of endometrial cells. Based on our findings, the conditioned medium of the activated macrophages could modify hepcidin synthesis via the nuclear factor kappa\u2010light\u2010chain\u2010enhancer of activated B cells, the signal transducer and activator of transcription 3, and the transferrin receptor 2\/bone morphogenetic protein 6\/suppressor of mothers against decapentaplegic 1\/5\/8 signaling pathways, and FKN could alter this effect on the endometrial cells. It was also revealed that the conditioned macrophage medium and FKN modulated the iron release and storage of HEC\u20101A cells. FKN signaling may be involved in the management of iron trafficking of the endometrium by the regulation of hepcidin. It can contribute to the iron supply for fetal development at the early stage of the pregnancy.<\/jats:p>","DOI":"10.1002\/cbin.12144","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T07:55:06Z","timestamp":1709020506000},"page":"737-754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Regulation of iron metabolism in HEC\u20101A endometrium cells by macrophage\u2010derived factors and fractalkine"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5012-3242","authenticated-orcid":false,"given":"Edina","family":"Pandur","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biology, Faculty of Pharmacy University of P\u00e9cs P\u00e9cs Hungary"},{"name":"National Laboratory on Human Reproduction University of P\u00e9cs P\u00e9cs 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