{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:17:47Z","timestamp":1791555467035,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Asthma is an inflammatory disease. Airway epithelial cell pyroptosis and cytokine secretion promote asthma progression. Tripartite motif 47 (TRIM47)\u00a0belongs to the E3 ubiquitin ligase family and is associated with apoptosis and inflammation in a range of diseases. However, the role of TRIM47 in asthma has not been explored. In this study, the human bronchial epithelial cell line BEAS\u20102B was treated with house dust mite (HDM) and TRIM47 expression was detected by RT\u2010qPCR and Western blot. After transfection with TRIM47 interfering and overexpressing plasmids, the synthesis and secretion of cytokines, as well as pyroptosis\u2010related indicators, were examined.\u00a0Nuclear factor kappa\u2010B (NF\u2010\u03baB)\u00a0pathway proteins and nod\u2010like receptor protein 3 (NLRP3)\u00a0inflammasome were measured to explore the mechanism of TRIM47 action. In addition, the effect of TRIM47 on the level of NF\u2010\u03baB essential modulator (NEMO) ubiquitination was detected by an immunoprecipitation assay. The results showed that TRIM47 was upregulated in HDM\u2010induced BEAS\u20102B cells and that TRIM47 mediated HDM\u2010induced BEAS\u20102B cell pyroptosis and cytokine secretion. Mechanistically, TRIM47 promoted the K63\u2010linked ubiquitination of NEMO and facilitated NF\u2010\u03baB\/NLRP3 pathway activation. In conclusion, TRIM47 may promote cytokine secretion mediating inflammation and pyroptosis in bronchial epithelial cells by activating the NF\u2010\u03baB\/NLRP3 pathway. Therefore, TRIM47 may be a potential therapeutic target for HDM\u2010induced asthma.<\/jats:p>","DOI":"10.1002\/cbin.12186","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T04:38:05Z","timestamp":1716266285000},"page":"1138-1147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["TRIM47 promotes HDM\u2010induced bronchial epithelial pyroptosis by regulating NEMO ubiquitination to activate NF\u2010\u03baB\/NLRP3 signaling"],"prefix":"10.1002","volume":"48","author":[{"given":"Wenjuan","family":"Zhan","sequence":"first","affiliation":[{"name":"Department of Emergency Xi'an Children's Hospital Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Xi'an Children's Hospital Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Emergency Xi'an Children's Hospital Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6667-884X","authenticated-orcid":false,"given":"Li","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Emergency Xi'an Children's Hospital Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-019-0079-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100784118"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.716469"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2070"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.10.012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.01.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3786"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-022-00909-1"},{"key":"e_1_2_9_10_1","first-page":"326","article-title":"PARK2 attenuates house dust mite\u2010induced inflammatory reaction, pyroptosis and barrier dysfunction in BEAS\u20102B cells by ubiquitinating NLRP3","volume":"13","author":"Ge X.","year":"2021","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.05.065"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.25"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42781"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12040401"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.03.018"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105429"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117893"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-02040-8"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-021-00277-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.004950"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-018-8693-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2500\/aap.2019.40.4253"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-022-00953-9"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.04.018"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1761-4"},{"key":"e_1_2_9_26_1","first-page":"757","article-title":"Der f1 induces pyroptosis in human bronchial epithelia via the NLRP3 inflammasome","volume":"41","author":"Tsai Y. 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