{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:54:56Z","timestamp":1789772096206,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We evaluated the influence of different media plus various concentrations of Glial cell line\u2010derived neurotrophic factor (GDNF) during the in vitro culture (IVC) of testicular tissues from prepubertal collared peccary. Testes from 5 individuals were collected, fragmented and cultured for 28 days (34\u00b0C and 5% CO<jats:sub>2<\/jats:sub>). Culture media were Dulbecco's modified essential medium (DMEM) or stem cell serum free media (StemPro\u201034\u2122 SFM), both supplemented with various concentrations of GDNF (0, 10, or 20\u2009ng\/mL). Fragments were cultured on the flat surface of 0.75% agarose gel and were evaluated every 7 days for fragment area, histomorphology, cellular viability, and proliferative activity. Data were expressed as mean\u2009\u00b1\u2009standard error and analyzed by Kruskal\u2013Wallis's and Tukey test. Fragments area decreased over the 28 days\u2010culture, regardless of the treatment. For morphology, the StemPro\u201037 SFM medium plus 10\u2009ng\/mL GDNF provided higher scores at all time points in comparison to DMEM using any GDNF concentration (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). After 28 days, similar cellular viability (~70%) was observed in all treatments (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). For proliferating cell nuclear antigen assay, only DMEM plus 10\u2009ng\/mL GDNF improved (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) cellular proliferation on Days 14 and 28. Looking at argyrophilic nucleolar organizing regions, after 28 days, there were no differences among treatments regarding cell proliferative capacity for both spermatogonia and Sertoli cells (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). In summary, the DMEM and StemPro\u201034 SFM are adequate medium for IVC of prepubertal peccary testicular tissue. Supplementation with GDNF, especially at a 10\u2009ng\/mL concentration, appears to be essential for the maintenance of cell survival and proliferation.<\/jats:p>","DOI":"10.1002\/cbin.12203","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T05:34:08Z","timestamp":1719034448000},"page":"1364-1377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Synergistic effects of glial cell line\u2010derived neurotrophic factor and base\u2010medium on in vitro culture of testicular tissue derived from prepubertal collared peccary"],"prefix":"10.1002","volume":"48","author":[{"given":"Andreia Maria da","family":"Silva","sequence":"first","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi\u2010Arid UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Gl\u00f3ria","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi\u2010Arid UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Graziele Pereira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi\u2010Arid UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreza Vieira","family":"Brasil","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi\u2010Arid UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2137-854X","authenticated-orcid":false,"given":"Alexsandra Fernandes","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Franco","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Morphology UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Ribeiro","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Oocyte and Preantral Ovarian Follicle Manipulation UECE Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy Vanessa Sulca","family":"\u00d1aupas","sequence":"additional","affiliation":[{"name":"Laboratory of Oocyte and Preantral Ovarian Follicle Manipulation UECE Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Comizzoli","sequence":"additional","affiliation":[{"name":"Smithsonian's National Zoo and Conservation Biology Institute Washington District of Columbia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-1062","authenticated-orcid":false,"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi\u2010Arid UFERSA Mossoro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.08.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-021-08099-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-021-00972-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1007\/s10815-020-01912-5","article-title":"EGF, GDNF, and IGF\u20101 influence the proliferation and stemness of ovine spermatogonial stem cells in vitro","volume":"37","author":"Binsila B. 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