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Human <jats:italic>JRK<\/jats:italic> DNA variants and gene expression levels are implicated in cancers and neuropsychiatric disorders. JRK protein modulates \u03b2\u2010catenin\u2013TCF activity but little is known of its cellular functions. Based on its homology to CENP\u2010B, we determined whether JRK binds centromeric or other satellite DNAs. We show that human JRK binds satellite III DNA, which is abundant at the chromosome 9q12 juxtacentromeric region and on Yq12, both sites of nuclear stress body assembly. Human JRK\u2010GFP overexpressed in HeLa cells strongly localises to 9q12. Using an anti\u2010JRK antiserum we show that endogenous JRK co\u2010localises with a subset of centromeres in non\u2010stressed cells, and with heat shock factor 1 following heat shock. Knockdown of JRK in HeLa cells proportionately reduces heat shock protein gene expression in heat\u2010shocked cells. A role for JRK in regulating the heat shock response is consistent with the mouse <jats:italic>Jrk<\/jats:italic> null phenotype and suggests that human <jats:italic>JRK<\/jats:italic> may act as a modifier of diseases with a cellular stress component.<\/jats:p>","DOI":"10.1002\/cbin.12216","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T09:08:41Z","timestamp":1719824921000},"page":"1212-1222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["JRK binds satellite III DNA and is necessary for the heat shock response"],"prefix":"10.1002","volume":"48","author":[{"given":"Rosalie","family":"Waldron","sequence":"first","affiliation":[{"name":"School of Biochemistry and Cell Biology University College Cork Cork Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de los Angeles 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