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To evaluate the role of PRKCQ in spermatogenesis, <jats:italic>Prkcq<\/jats:italic> knockout mice were generated using CRISPR\/Cas9 system. Histological and immunofluorescence assays by different markers were employed to assess the testicular cells variation. Sperm parameters were analyzed by computer\u2010assisted sperm analyzer. qPCR assay was used to examine the expression levels of other PKC family genes. We found that PRKCQ was conserved throughout evolution and highly expressed in testis. <jats:italic>Prkcq<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice were successfully generated and developed viably. Normal fertility was observed in <jats:italic>Prkcq<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> males. <jats:italic>Prkcq<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice exhibited no defects in spermatogenic cells and mature sperm were full in epididymis. Furthermore, there were no differences in sperm motility and progressive motility between <jats:italic>Prkcq<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> males and controls. Our findings report a detailed phenotypic analysis of <jats:italic>Prkcq<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> males and indicate that PRKCQ is not required for spermatogenesis in male mice, which can provide basic information for other researchers.<\/jats:p>","DOI":"10.1002\/cbin.70014","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T21:19:27Z","timestamp":1741727967000},"page":"522-533","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["PRKCQ Is Dispensable for Spermatogenesis in Mice"],"prefix":"10.1002","volume":"49","author":[{"given":"Yu","family":"Chen","sequence":"first","affiliation":[{"name":"Medical Research Center, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Center Nanjing Medical University Changzhou China"},{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology Nanjing 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