{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:54:53Z","timestamp":1766159693296,"version":"3.41.2"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Current osteoporosis treatments are insufficient as they cause a relatively small increase in bone mass and are unable to recover lost bone structures, in addition to having severe side effects. The bone morphogenetic protein (BMP) and Wnt\/\u03b2\u2010catenin signaling pathways cooperatively modulate bone formation and osteoblast differentiation and therefore may play a role in treating osteoporosis. This study aimed to investigate the effects of Ishophloroglucin A (IPA), a novel phenolic compound isolated from <jats:italic>Ishige okamurae<\/jats:italic>, on osteoblast differentiation by activating the BMP and Wnt\/\u03b2\u2010catenin signaling pathways. According to our findings, IPA significantly promoted the osteogenic proliferation of MC3T3\u2010E1 osteoblastic cells and increased alkaline phosphatase (ALP) activity and calcium nodule formation in MC3T3\u2010E1 cells compared to the untreated control. IPA also upregulated osteogenesis markers such as type 1 collagen, ALP, p\u2010Smad1\/5\/8, osterix, osteopontin, runt\u2010related transcription factors (Runx2), and BMP2 in MC3T3\u2010E1 cells in a dose\u2010dependent manner. Moreover, IPA activated Wnt3a, LRP5, DVL2, and \u03b2\u2010catenin in MC3T3\u2010E1 cells. Overall, our results demonstrate that IPA promotes the differentiation of MC3T3\u2010E1 osteoblastic cells by activating the BMP and Wnt\/\u03b2\u2010catenin signaling pathways, suggesting that it may be a potential candidate target for treating or preventing osteoporosis.<\/jats:p>","DOI":"10.1002\/cbin.70030","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T09:49:48Z","timestamp":1746697788000},"page":"965-974","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ishophloroglucin A Isolated From <i>Ishige okamurae<\/i> Stimulates Osteoblast Differentiation Through Activation of the Bone Morphogenetic Protein and Wnt\/\u03b2\u2010Catenin Signaling Pathways in MC3T3\u2010E1 Cells"],"prefix":"10.1002","volume":"49","author":[{"given":"Seung\u2010Hong","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Engineering Soonchunhyang University Asan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihyang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, College of Medical and Life Science Silla University Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Im","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, College of Medical and Life Science Silla University Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang\u2010Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Research Center for Extremophiles and Marine Microbiology Silla University Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3123-2870","authenticated-orcid":false,"given":"Mi Hwa","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, College of Medical and Life Science Silla University Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmhi.2012.09.002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.01.024"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.24875\/BMHIM.21000171"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3074"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2016.06.007"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1326-0"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2020.1836606"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.2929"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1834"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2017.1290085"},{"key":"e_1_2_11_12_1","first-page":"313","article-title":"In Vitro Antibacterial and Anti\u2010Inflammatory Properties of Seaweed Extracts Against Acne Inducing Bacteria, Propionibacterium acnes","volume":"32","author":"Choi J. 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