{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T03:28:17Z","timestamp":1786678097903,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Colonization of <jats:italic>Helicobacter pylori<\/jats:italic> (H. pylori) in stomach often causes gastritis, an inflammation of the stomach lining that is closely associated with serious conditions like ulcers and gastric cancer. Of the toxicity mechanisms, microbial lipopolysaccharide (LPS) binding to TLR4 receptor on the glandular cells activates the NF\u2010\u03baB pathway, inducing pro\u2010inflammatory cytokine release and immune cell infiltration, which results in the tissue damage. However, whether LPS has any direct damaging effect on gastric epithelial cells has been in debate. By using mouse gastric organoids that were grown from the isolated glands and maintained the cellular compositions, we demonstrate various effects of variable LPS concentrations on the glandular epithelial cells, including mild promotion of cell proliferation at the low concentration and induced loss of cell polarity and altered gene expressions at the high concentration. These findings provide insights into how LPS directly affects the stomach lining.<\/jats:p>","DOI":"10.1002\/cbin.70066","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T08:31:07Z","timestamp":1754382667000},"page":"1454-1463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Modeling Lipopolysaccharide\u2010Elicited Inflammation Using 3D Mouse Gastric Organoids"],"prefix":"10.1002","volume":"49","author":[{"given":"Aoqing","family":"Xu","sequence":"first","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifan","family":"Ye","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4512-2215","authenticated-orcid":false,"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengrui","family":"Yang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"McKay","family":"Mullen","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7480-5085","authenticated-orcid":false,"given":"Xing","family":"Liu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8565-6011","authenticated-orcid":false,"given":"Zhikai","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics University of Science and Technology of China Hefei Anhui China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale Hefei Anhui China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2707"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01326"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0898-6568(00)00149-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13619-024-00218-6"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-019-0178-z"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/019262330002800210"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07291-6"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjae030"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006-291x(05)80040-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.011746"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2008.01.006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(84)91816-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-021-00436-w"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405979102"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-021-00875-7"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abp9020"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800527200"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202200263"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjac007"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2024.101207"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjaa039"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.02.036"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.165340"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.507970"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.70066","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T14:11:36Z","timestamp":1760451096000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.70066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,5]]},"references-count":24,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/cbin.70066"],"URL":"https:\/\/doi.org\/10.1002\/cbin.70066","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,5]]},"assertion":[{"value":"2025-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}