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Paclitaxel, a widely used chemotherapeutic agent for non\u2010small cell lung cancer (NSCLC), often faces resistance that limits its clinical efficacy. Therefore, identifying molecular markers that modulate paclitaxel responsiveness is critical. The ubiquitin\u2010proteasome system (UPS), which regulates protein homeostasis, plays a role in cancer progression, apoptosis, and drug resistance, with deubiquitinating enzymes (DUBs), serving as key regulators. Recent studies suggest that targeting specific DUBs may enhance drug sensitivity. This study aimed to investigate the expression patterns of <jats:italic>DUB<\/jats:italic> genes in response to paclitaxel treatment. Multiplex RT\u2010PCR and RT\u2010qPCR analysis revealed that <jats:italic>USP1<\/jats:italic>, <jats:italic>USP5<\/jats:italic>, <jats:italic>USP28<\/jats:italic>, and <jats:italic>USP34<\/jats:italic> were downregulated, whereas <jats:italic>USP10<\/jats:italic> and <jats:italic>USP36<\/jats:italic> were upregulated in paclitaxel\u2010treated A549 cells. Western blot analysis confirmed changes in protein levels consistent with mRNA expression for all DUBs except USP10 and USP36, which displayed discordant patterns. Furthermore, paclitaxel\u2010induced apoptosis was verified by altered levels of apoptotic and antiapoptotic proteins including PARP, caspase\u20103, Bax, Bcl\u20102, Bcl\u2010XL, and p53. The identification of these <jats:italic>DUB<\/jats:italic> genes highlights their potential as biomarkers for predicting drug responsiveness and prognosis during paclitaxel treatment, thereby proposing a new direction for improving the therapeutic efficacy of paclitaxel in NSCLC.<\/jats:p>","DOI":"10.1002\/cbin.70072","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T05:19:02Z","timestamp":1756099142000},"page":"1507-1517","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression Patterns of Deubiquitinating Enzymes in Paclitaxel\u2010Treated Lung Cancer Cells"],"prefix":"10.1002","volume":"49","author":[{"given":"Hwa\u2010Yeong","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biomedical Science CHA University Gyeonggi\u2010Do Republic of 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