{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:22:13Z","timestamp":1776298933202,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Chagas disease, caused by the protozoan <jats:italic>Trypanosoma cruzi<\/jats:italic>, is a neglected tropical disease with limited treatment options and no available vaccine. Understanding the role of proteins in the parasite's biological cycle is critical for advancing vaccine development and optimizing therapies. The flagellar attachment zone (FAZ) proteins play a pivotal role in motility, pathogenicity, and cell division in trypanosomatids, but their functions in <jats:italic>T. cruzi<\/jats:italic> are not as well\u2010characterized as in <jats:italic>Trypanosoma brucei<\/jats:italic> and <jats:italic>Leishmania spp<\/jats:italic>. This study investigates the orthologous TcGP72 and TcFLA\u20101BP proteins in <jats:italic>T. cruzi<\/jats:italic>, focusing on their roles in the infective forms of the parasite. Our findings demonstrate that TcFLA\u20101BP is important for efficient host cell infection in vitro, indicating its critical role in the parasite\u2032s infectivity. Conversely, TcGP72 is nonessential for the infection process, but significantly contributes to cytoskeletal remodeling during the parasite\u2032s life cycle. These results provide new insights into the distinct functional roles of FAZ proteins in <jats:italic>T. cruzi<\/jats:italic>. Furthermore, the study underscores the importance of TcGP72 in maintaining cellular architecture, reinforcing the relevance of FAZ proteins in the parasite\u2032s pathogenesis and structural integrity.<\/jats:p>","DOI":"10.1002\/cbin.70076","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T12:54:25Z","timestamp":1756904065000},"page":"1395-1410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of TcFLA\u20101BP and TcGP72 Deletion on the Infectivity and Survival of <i>Trypanosoma cruzi<\/i> in Cell Cultures"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2025-1088","authenticated-orcid":false,"given":"Normanda","family":"Souza\u2010Melo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisas em Medicina de Precis\u00e3o Instituto de Biof\u00edsica Carlos Chagas Filho\u2014 Universidade Federal do Rio de Janeiro, Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Biomagens, Centro Nacional de Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Av. Carlos Chagas Filho, 373, Centro de Ci\u00eancias da Sa\u00fade Cidade Universit\u00e1ria, Ilha do Fund\u00e3o, Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna Henriques","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisas em Medicina de Precis\u00e3o Instituto de Biof\u00edsica Carlos Chagas Filho\u2014 Universidade Federal do Rio de Janeiro, Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Biomagens, Centro Nacional de Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Av. Carlos Chagas Filho, 373, Centro de Ci\u00eancias da Sa\u00fade Cidade Universit\u00e1ria, Ilha do Fund\u00e3o, Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanderley","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisas em Medicina de Precis\u00e3o Instituto de Biof\u00edsica Carlos Chagas Filho\u2014 Universidade Federal do Rio de Janeiro, Instituto Nacional de Ci\u00eancia e Tecnologia em Biologia Estrutural e Biomagens, Centro Nacional de Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil"},{"name":"Centro de Estudos Biom\u00e9dicos\u2010CMABio, Escola Superior de Sa\u00fade Universidade do Estado do Amazonas\u2010UEA, Manaus, Av. 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