{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:19:56Z","timestamp":1782940796152,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Advances in Assisted Reproductive Technologies (ARTs) help overcome reproductive barriers. However, a comprehensive understanding of embryonic development is crucial for their success.\n                    <jats:italic>In vitro<\/jats:italic>\n                    fertilization (IVF) and artificial oocyte activation (AOA) are embryo production methods commonly used. Previous studies have reported low developmental success with these methods, possibly due to the culture medium used. Therefore, we aimed to optimize ARTs in red\u2010rumped agouti by evaluating the influence of production method and culture medium on embryonic development. Oocytes were matured in vitro and divided into two groups: IVF with capacited spermatozoa or AOA with strontium chloride and cytochalasin B. Presumed zygotes were cultured in either KSOM or SOF medium after a 6\u2010h incubation. Morphology, oocyte\u2010sperm interaction, developmental kinetics, and oxidative stress levels were assessed. IVF\u2010derived structures exhibited a higher rate of normal morphology compared to AOA\u2010derived structures (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Embryo kinetics analysis showed that AOA\u2010KSOM and IVF\u2010KSOM groups had a higher number of cleaved structures than the SOF groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) on Day 2. On Day 5, the IVF\u2010KSOM group presented the highest percentage of cleavage\/total zygotes and the highest percentage of structures with eight or more cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Morula formation was significantly higher in IVF\u2010KSOM and AOA\u2010KSOM (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), highlighting KSOM's effectiveness. Notably, only the IVF\u2010KSOM group produced blastocyst (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Oxidative stress assessment showed no differences between groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). These findings confirm that IVF\u2010KSOM is the most effective methodology for developing red\u2010rumped agouti embryos, offering valuable insights into the reproductive biology of this species and aiding in the refinement of protocols for closely related rodents.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70080","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T11:37:04Z","timestamp":1757590624000},"page":"1655-1668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of Embryo Production Method and Culture Medium on Embryonic Development in Red\u2010Rumped Agouti"],"prefix":"10.1002","volume":"49","author":[{"given":"Lhara Ricarliany Medeiros de","family":"Oliveira","sequence":"first","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Vitorino Costa de","family":"Aquino","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Grasiele Pereira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moacir Franco de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Morphophysiology Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6027-1062","authenticated-orcid":false,"given":"Alexandre Rodrigues","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Germplasm Conservation Federal Rural University of Semi\u2010Arid Mossor\u00f3 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2137-854X","authenticated-orcid":false,"given":"Alexsandra Fernandes","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Biotechnology Federal Rural University of Semi\u2010Arid Mossor\u00f3 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