{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T08:21:32Z","timestamp":1762935692638,"version":"3.45.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the cytotoxic and genotoxic potential of lercanidipine hydrochloride (LHC) on human peripheral lymphocytes using in vitro and in silico approaches. The micronucleus (MN) test revealed a significant, dose\u2010dependent increase in MNs, particularly after 24\u2010h treatment (***\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), which suggests potential DNA damage. However, the chromosome aberration (CA) test did not yield statistically significant results, although a dose\u2010related upward trend was observed. A substantial reduction in both the mitotic index (MI) and the nuclear division index (NDI) was observed at all concentrations (**\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01, ***\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), indicating strong cytotoxic effects. In silico molecular docking analysis revealed that the ligand (LHC) binds to the minor groove of B\u2010DNA with a binding energy of \u22127.7\u2009kcal\/mol, forming non\u2010covalent interactions similar to those of the known minor groove binder Netropsin (\u22128.14\u2009kcal\/mol). By contrast, the positive control, mitomycin C (MMC), binds to the major groove via intercalation, forming covalent cross\u2010links, with a binding energy of \u22125.45\u2009kcal\/mol. Although LHC's minor groove binding suggests a potential preference for AT\u2010rich regions, its sequence specificity remains unconfirmed. Overall, these findings suggest that LHC primarily exhibits cytotoxic effects (e.g., inhibition of DNA replication or transcription), with only limited and preliminary evidence of genotoxic potential. Further studies are needed to elucidate the safety of LHC and its DNA interaction mechanisms.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70083","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T08:30:27Z","timestamp":1758875427000},"page":"1682-1691","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytogenotoxic Evaluation of Lercanidipine Hydrochloride in Human Lymphocytes Using Micronucleus, Chromosome Abnormalities, and Molecular Docking Assays"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9848-954X","authenticated-orcid":false,"given":"\u0130brahim Halil","family":"Kenger","sequence":"first","affiliation":[{"name":"Department of Medical Genetics Gaziantep Islam Science and Technology University Gaziantep Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7647-6556","authenticated-orcid":false,"given":"Ahmet","family":"Kard\u00f6l","sequence":"additional","affiliation":[{"name":"Department of Biology Kahramanmara\u015f S\u00fct\u00e7\u00fc \u0130mam University Kahramanmara\u015f Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Kayraldiz","sequence":"additional","affiliation":[{"name":"Department of Biology Kahramanmara\u015f S\u00fct\u00e7\u00fc \u0130mam University Kahramanmara\u015f Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1424-5132","authenticated-orcid":false,"given":"Mehmet Tahir","family":"H\u00fcsunet","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Gaziantep Islam Science and Technology University Gaziantep Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8341-4332","authenticated-orcid":false,"given":"Tu\u011f\u00e7e Nur","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Biology Kahramanmara\u015f S\u00fct\u00e7\u00fc \u0130mam University Kahramanmara\u015f Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40203-023-00164-2"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.18663\/tjcl.331245"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(92)90038-4"},{"issue":"24","key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.3390\/ani14243674","article-title":"The Relationship Between Oxidative Stress and Infertility Due to Antihypertensive Drugs in Rattus norvegicus","volume":"14","author":"Asir B.","year":"2024","journal-title":"Animals : An Open Access Journal From MDPI"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2022.2026375"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2005.12.002"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2009.04.056"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.23736\/S0392-0488.18.05870-4"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002040000187"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0959-440x(03)00081-2"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573407215666191102130417"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.2.315"},{"key":"e_1_2_12_14_1","doi-asserted-by":"crossref","unstructured":"Evans H.1984. 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