{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T07:47:50Z","timestamp":1768636070362,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We previously showed that inhibition of the NF\u2010\u03baB signaling pathway in mesenchymal stromal cells (MSCs) (NKI\u2010MSCs) induces quiescence in co\u2010cultured hematopoietic stem cells (HSCs). This led us to investigate whether NKI\u2010MSCs exert similar growth\u2010inhibitory effects on leukemic cells. We found that both NKI\u2010MSCs and their secretome induce cell cycle arrest in KG1a cells, a cell line of acute myeloid leukemia (AML) origin. Surprisingly, the extracellular vesicles (EVs) isolated from NKI\u2010MSCs supported the proliferation of KG1a cells. This is perhaps the first report showing the opposite effects of MSCs and the EVs secreted by them. Further analysis revealed that microvesicles (MVs) from NKI\u2010MSCs inhibited KG1a cell growth and induced apoptosis, whereas exosomes (Exos) supported proliferation. Our findings could have clinical implications. NKI\u2010MVs, having apoptosis\u2010inducing activity, could serve as an adjunct, off\u2010the\u2010shelf biologic to limit AML growth, enabling reduced\u2010intensity chemotherapy in elderly patients and patients having co\u2010morbidities. NF\u2010\u03baB inhibitors have been tried as chemotherapeutic agents for treating AML patients. However, systemic inhibition of NF\u2010\u03baB may also affect the bone marrow resident MSCs, which in turn could produce EVs supporting the proliferation of AML blasts. Our data could explain the inadequate clinical effectiveness of NF\u2010\u03baB inhibitors in treating AML, and also raise a concern for the systemic use of NF\u2010\u03baB inhibitors in the therapeutic regimen.<\/jats:p>","DOI":"10.1002\/cbin.70113","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T15:53:41Z","timestamp":1765986821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pharmacological Inhibition of NF\u2010\u03baB in Mesenchymal Stromal Cells Selectively Partitions Apoptosis\u2010Inducing Factors in Their Microvesicles and Proliferation\u2010Inducing Factors in Their Exosomes: Implications in the Management of Acute Myeloid Leukemia"],"prefix":"10.1002","volume":"50","author":[{"given":"Shalmali","family":"Pendse","sequence":"first","affiliation":[{"name":"Symbiosis School of Biological Sciences Symbiosis International (Deemed University) Pune 412115 India"},{"name":"Symbiosis Centre for Stem Cell Research Symbiosis International (Deemed University) Pune 412115 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayali","family":"Chavan","sequence":"additional","affiliation":[{"name":"Symbiosis School of Biological Sciences Symbiosis International (Deemed University) Pune 412115 India"},{"name":"Symbiosis Centre for Stem Cell Research Symbiosis International (Deemed University) Pune 412115 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishakha","family":"Kasherwal","sequence":"additional","affiliation":[{"name":"Symbiosis School of Biological Sciences Symbiosis International (Deemed University) Pune 412115 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7498-7563","authenticated-orcid":false,"given":"Vaijayanti","family":"Kale","sequence":"additional","affiliation":[{"name":"Symbiosis Centre for Stem Cell Research Symbiosis International (Deemed University) Pune 412115 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuradha","family":"Vaidya","sequence":"additional","affiliation":[{"name":"Symbiosis School of Biological Sciences Symbiosis International (Deemed University) Pune 412115 India"},{"name":"Symbiosis Centre for Stem Cell Research Symbiosis International (Deemed University) Pune 412115 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2020.11.009"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-021-10240-9"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.119.259796"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.663316"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33861-1"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14143557"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/08820139.2020.1712416"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2912"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2017.5729"},{"key":"e_1_2_13_11_1","doi-asserted-by":"crossref","unstructured":"Kale V.2023.Priming of Human Bone Marrow\u2010derived Mesenchymal Stromal Cells With Various Down\u2010stream Effectors of the Transforming Growth Factor 1 Pathway Boosts Their Hematopoiesis\u2010Supportive Ability.https:\/\/doi.org\/10.22541\/au.168595395.52538091\/v1.","DOI":"10.22541\/au.168595395.52538091\/v1"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2025.106734"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11437"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11904"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.149"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01735-1"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0373-9"},{"key":"e_1_2_13_18_1","doi-asserted-by":"crossref","unstructured":"Leimk\u00fchler N. 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