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However, the developmental process of ICCs and their role in intestinal development remain unclear. In this study, we analyzed single\u2010cell transcriptome data and found that\n                    <jats:italic>odd\u2010skipped\u2010related 1 (OSR1)<\/jats:italic>\n                    is expressed in the intestinal tissues of humans and zebrafish. Additionally,\n                    <jats:italic>OSR1<\/jats:italic>\n                    expression was reduced in tissue biopsies obtained from IBD patients. To further explore the function of\n                    <jats:italic>osr1<\/jats:italic>\n                    in intestinal development and disease, we generated transgenic lines\n                    <jats:italic>Tg(osr1:mCherry)<\/jats:italic>\n                    and\n                    <jats:italic>Tg(osr1:eGFP)<\/jats:italic>\n                    . Furthermore, we found that these transgenic fish could label interstitial cells of Cajal\u2010like cells (ICC\u2010like cells) from 48\u2009h post\u2010fertilization (hpf) to adulthood.\n                    <jats:italic>osr1<\/jats:italic>\n                    \u2010positive ICC\u2010like cells form a network structure that envelops the intestine and coordinates its development. Moreover, mutations in\n                    <jats:italic>osr1<\/jats:italic>\n                    lead to developmental defects in the intestine, characterized by disrupted ICC\u2010like cells network structure, intestinal wall hypoplasia, and increased intestinal permeability, potentially mediated through the regulation of\n                    <jats:italic>yap1<\/jats:italic>\n                    . Overall, our findings indicate that\n                    <jats:italic>osr1<\/jats:italic>\n                    acts as a novel marker gene for ICC\u2010like cells and plays a crucial role in intestinal development.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70162","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T09:32:32Z","timestamp":1778751152000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>osr1<\/i>\n                    as a Novel Marker for ICC\u2010Like Cells Development in Zebrafish"],"prefix":"10.1002","volume":"50","author":[{"given":"Wenmin","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqin","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"He","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Medicine, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing P.R. China"},{"name":"Department of Ultrasound Medicine Affiliated Hospital of North Sichuan Medical College Chongqing P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongwei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TingTing","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the Key Laboratory for the Prevention and Treatment of Kidney Disease of Chongqing, Chongqing Clinical Research Center of Kidney and Urology Diseases, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing P.R. 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