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Due to the limited availability of experimental models, understanding distinct molecular pathways and potential therapeutic targets remains challenging. This study aimed to establish patient\u2010derived cell cultures (PDCs) from IMPC to generate viable models for in\u2010vitro studies. Tissue samples from five IMPC cases were enzymatically disaggregated using five different cell disaggregation protocols. These cells were characterized using immunofluorescence, short tandem repeats profiling, and real\u2010time assays for tumor marker expression profiles. RNA sequencing was performed and compared with invasive ductal carcinoma, no special type (IDC\u2010NST), to study differential gene expression and cell polarity markers. Two short\u2010term PDCs were successfully established from IMPC samples with an optimized collagenase\u2010based protocol. These cultures showed an immunohistochemical profile consistent with the original tumor tissues and maintained hormone receptor and MUC1 expression status. RNA sequencing of PDCs revealed similar gene expression patterns with matched tumor tissue and revealed upregulated RAP1, MAPK, and PI3K\u2010AKT pathways, when compared with ER\/PR\u2010matched IDC. These PDCs also showed different gene expression patterns in cell\u2010polarity associated genes, such as cadherins\n                    <jats:italic>CDH2<\/jats:italic>\n                    , tight junction gene\n                    <jats:italic>MARVELD2<\/jats:italic>\n                    , PAR complex gene\n                    <jats:italic>PARD6B<\/jats:italic>\n                    , and downregulation of the cell polarity\n                    <jats:italic>CRB2<\/jats:italic>\n                    gene. This study indicated the need for an optimization of cell culture conditions and the feasibility of establishing patient\u2010derived cell cultures from IMPC. These models provide a great tool to study molecular insights, cell polarity, and therapeutic research in a rare breast cancer subtype.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70167","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T11:46:58Z","timestamp":1779536818000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and Molecular Characterization of a Short\u2010Term Primary Culture Derived From Invasive Micropapillary Carcinoma of the Breast"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8618-6314","authenticated-orcid":false,"given":"Mamta","family":"Gurav","sequence":"first","affiliation":[{"name":"Molecular Pathology Laboratory, Department of Pathology Tata Memorial Hospital Mumbai Maharashtra India"},{"name":"Homi Bhabha National Institute Training School Complex Mumbai Maharashtra 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