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The present study investigates the influence of tamoxifen on actin stress fibre organisation in breast cancer cells. We demonstrate that tamoxifen treatment induces significant morphological changes, including increased cell spreading and polarity and enhanced migratory potential in breast cancer cells T47D and ZR\u201075\u20101. A loss of organised actin stress fibres and the emergence of lamellipodia and filopodia by tamoxifen treatment indicate its direct role in actin cytoskeleton remodelling, which was further confirmed by western blot analyses of key actin regulators. Upstream tamoxifen activates c\u2010Src kinase, while reducing phosphorylation of myosin light chain (MLC), a key stress fibre regulator that activates myosin II. Further, inhibition of c\u2010Src kinase with PP2 or silencing using siRNA reversed tamoxifen\u2010induced stress fibre loss, confirming c\u2010Src's pivotal role in modulating actin dynamics. These findings highlight a novel mechanism by which tamoxifen influences breast cancer cell actin dynamics and motility.<\/jats:p>","DOI":"10.1002\/cbin.70169","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T13:21:12Z","timestamp":1779801672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tamoxifen Modulates Actin Stress Fibre Dynamics Through c\u2010Src\u2014MLCK Signalling in Breast Cancer Cells"],"prefix":"10.1002","volume":"50","author":[{"given":"Bidisha","family":"Biswas","sequence":"first","affiliation":[{"name":"Department of Biotechnology National Institute of Pharmaceutical Education and Research \u2013 Guwahati Guwahati India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahnawaz","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Biotechnology National Institute of Pharmaceutical Education and Research \u2013 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