{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:58Z","timestamp":1786977298820,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gastric mucosal inflammation is a critical precondition of various gastric diseases, and development of safe and effective anti\u2010inflammatory agents for prompt resolution of acute inflammation at early stage is vital to preventive medicine. B vitamins are essential water\u2010soluble nutrients with reported anti\u2010inflammatory properties, yet their potent regulatory effects in gastric mucosal inflammation are not systemically assessed. In this study, we adopted a lipopolysaccharide (LPS)\u2010induced inflammatory mouse gastric organoid model to screen for anti\u2010inflammatory B vitamin family members. With the anti\u2010inflammatory effect of vitamin B12 (VB12) being validated in this gastric organoid model, which is consistent with the roles in other biological settings, further screening of the rest B vitamin members identified vitamin B6 (VB6) as the most potent anti\u2010inflammatory agent as it preventatively ameliorated LPS\u2010induced organoid collapse and preserved gastric epithelial polarity in the inflammatory organoids. Functional assays confirmed that VB6 significantly downregulated the expressions of key pro\u2010inflammatory\n                    <jats:italic>Tnf<\/jats:italic>\n                    ,\n                    <jats:italic>Cxcl1<\/jats:italic>\n                    , and\n                    <jats:italic>Il1b<\/jats:italic>\n                    genes, and inhibited TNF\u2010\u03b1 and IL\u20101\u03b2 protein secretions. Further mechanistic exploration revealed that VB6 antagonized LPS\u2010induced inflammatory responses by targeting the transcription of positive regulatory genes in core LPS\u2010mediated inflammatory signaling pathways. These findings provide important experimental evidence for the development of nutrition\u2010based intervention strategies for gastric inflammatory diseases in human.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70172","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:14:11Z","timestamp":1780395251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Vitamin B6 Ameliorates Lipopolysaccharide\u2010Induced Inflammation in Mouse Gastric Organoids"],"prefix":"10.1002","volume":"50","author":[{"given":"Aoqing","family":"Xu","sequence":"first","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics &amp; Hefei National Research Center for Interdisciplinary Sciences at the Microscale University of Science and Technology of China Hefei China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijin","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Clinical Research Center for Cancer Sun Yat\u2010sen University Cancer Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyu","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics &amp; Hefei National Research Center for Interdisciplinary Sciences at the Microscale University of Science and Technology of China Hefei China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics &amp; Hefei National Research Center for Interdisciplinary Sciences at the Microscale University of Science and Technology of China Hefei China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengrui","family":"Yang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics &amp; Hefei National Research Center for Interdisciplinary Sciences at the Microscale University of Science and Technology of China Hefei China"},{"name":"Anhui Key Laboratory for Chemical Biology and New Quality Medicine Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Beijing University of Chinese Medicine Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4354-3796","authenticated-orcid":false,"given":"Chuanhai","family":"Fu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics &amp; 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