{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T07:52:06Z","timestamp":1781855526086,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Platelet shortages and limited storage stability restrict global platelet transfusion capacity, highlighting the need for efficient in vitro platelet production systems. This study establishes a simple, non\u2010genetic and cost\u2010effective system for efficient in vitro platelet\u2010like particle (PLP) production from human hematopoietic stem\/progenitor cells (HSPCs) using a Phase\u2010specific modulation of Hippo\u2010YAP\/TAZ signaling modulation. Temporal control of Hippo\u2010YAP\/TAZ signaling by lysophosphatidic acid (LPA), an activator of YAP\/TAZ activity, significantly enhanced megakaryocyte differentiation, expansion and PLP production, resulting in an approximately 15\u2010fold increase in PLP yield at the end of the procedure. Furthermore, LPA extended the expansion period of HSPC\u2010derived megakaryocytes up to 8 days, resulting in a greater than 20\u2010fold increase in the number of HSPC\u2010derived CD41\n                    <jats:sup>+<\/jats:sup>\n                    megakaryocytes. Moreover, replacement of expensive commercial recombinant human thrombopoietin (C\u2010rhTPO), one of the major cost\u2010driving components in in vitro PLP production, with recombinant human thrombopoietin produced in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (W\u2010rhTPO) further improved the cost\u2010effectiveness of the procedure. In conclusion, this study demonstrates that temporally controlled Hippo\u2010YAP\/TAZ signaling, together with affordable cytokine supplementation, provides a robust and GMP\u2010compatible platform for large\u2010scale PLP manufacturing for future clinical applications. We believe that this system will enable scalable PLP generation, even in resource\u2010constrained settings, to increase human platelet supply for many life\u2010saving therapies in the future.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70177","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:51:19Z","timestamp":1781542279000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Temporal Lysophosphatidic Acid Supplementation Enhances Megakaryocyte Differentiation and Platelet Production From Human Hematopoietic Progenitors"],"prefix":"10.1002","volume":"50","author":[{"given":"Nittaya","family":"Jiamvoraphong","sequence":"first","affiliation":[{"name":"Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkok 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