{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:28:34Z","timestamp":1787059714335,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Berberine (BBR), a natural alkaloid, exhibits potent anti\u2010tumor activity in colorectal cancer (CRC), but its mechanisms are not fully elucidated. Analysis of GEO datasets (GSE216908 and GSE184414) identified WDR54 as a key target of BBR treatment. In vitro, BBR treatment of CRC cell lines (HT\u201029 and SW480) markedly inhibited cell proliferation, DNA synthesis, and colony formation. Notably, BBR triggered G1\u2010phase cell cycle arrest and promoted apoptosis, as validated by flow cytometry and molecular marker analysis. WDR54 overexpression partially rescued these anti\u2010tumor effects, confirming its functional importance. Mechanistically, cellular thermal shift assays (CETSA) and mRNA stability assays revealed that BBR suppresses WDR54 expression via indirect transcriptional inhibition rather than direct protein binding or altered mRNA stability. Dual\u2010luciferase reporter assays further confirmed that BBR dose\u2010dependently inhibits WDR54 promoter activity. In vivo, an AOM\/DSS\u2010induced CRC mouse model showed that BBR reduced tumor number and size, alleviated pathological progression, and suppressed the WDR54\/\u03b2\u2010catenin axis. These findings suggest that BBR exerts significant anti\u2010tumor effects in CRC by downregulating WDR54 and inhibiting Wnt\/\u03b2\u2010catenin signaling, highlighting WDR54 as a potential therapeutic target and BBR as a promising treatment for CRC.<\/jats:p>","DOI":"10.1002\/cbin.70196","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:38:52Z","timestamp":1786448332000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Berberine Inhibited Tumor Growth Through Suppressing the WDR54\/\u03b2\u2010catenin Signal Pathway in Colorectal Cancer"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4636-7305","authenticated-orcid":false,"given":"Qin","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Proctology Kunming Municipal Hospital of Traditional Chinese Medicine Kunming China"},{"name":"Department of Proctology The Third 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