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Since miR\u2010374b and miR\u2010326 regulate cell differentiation, this study investigated if ADR\u03b22 regulates myoblast differentiation via miR\u2010374b\u20105p and miR\u2010326\u20103p. Primary myoblasts from ADR\u03b22 knockout (\u03b22KO) mice were cultured in proliferation and differentiation media. In silico, cellular and molecular analyses were performed to evaluate NF\u2010\u03baB signaling activation and validate mRNAs as miR\u2010374b\u20105p and miR\u2010326\u20103p targets in \u03b22KO differentiating myoblasts. Differentiating myoblasts from \u03b22KO mice had decreased mRNA levels of\n                    <jats:italic>Nr4a1, Myog, Myh3, Cdkn1c,<\/jats:italic>\n                    and\n                    <jats:italic>Tnni2<\/jats:italic>\n                    and expression of miR\u2010374b\u20105p and miR\u2010326\u20103p. Overexpression of these miRs increased fusion index and gene expression of myogenic markers of these cells. In silico analyses indicated that GSK\u20103\u03b2 and receptor activator of NF\u2010kB (Tnfrsf11a) had potential binding sites for miR\u2010374b\u20105p and miR\u2010326\u20103p, respectively. The dual\u2010luciferase reporter assay showed that\n                    <jats:italic>GSK\u20103\u03b2<\/jats:italic>\n                    and\n                    <jats:italic>Tnfrsf11a<\/jats:italic>\n                    were targets of miR\u2010374b\u20105p and miR\u2010326\u20103p, respectively. Regarding miR\u2010326\u20103p, there was a reduction of NF\u2010\u03baB signaling and\n                    <jats:italic>NF\u2010\u03baB<\/jats:italic>\n                    mRNA levels in differentiating myoblasts from \u03b22KO mice, and an upregulation of\n                    <jats:italic>Tnfrsf11a<\/jats:italic>\n                    and a downregulation of\n                    <jats:italic>Tnfrsf11<\/jats:italic>\n                    . In summary, ADR\u03b22 modulates myoblast differentiation via miR\u2010374b\u20105p\/\n                    <jats:italic>GSK\u20103\u03b2<\/jats:italic>\n                    and miR\u2010326\u20103p\/\n                    <jats:italic>Tnfrsf11a<\/jats:italic>\n                    axes.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70214","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:22:02Z","timestamp":1790410922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\u03b22 Adrenoceptor Signaling Modulates Myoblast Differentiation via miR\u2010374b\u20105p\/GSK3\u03b2 and miR\u2010326\u20103p\/Tnfrsf11a Axes"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9423-9965","authenticated-orcid":false,"given":"Tatiana E.","family":"Koike","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0567-5432","authenticated-orcid":false,"given":"Cesar S.","family":"Fuziwara","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Department of Biochemistry Federal University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrei R.","family":"Santos","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0700-2290","authenticated-orcid":false,"given":"Fernanda V. 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