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CENP\u2010A deposition is tightly regulated by assembly factors, including HJURP; however, the complete regulatory network remains incompletely defined. Using CENP\u2010A immunoprecipitation coupled with mass spectrometry, we identified SET, a multifunctional chromatin\u2010associated protein involved in transcription and nucleosome assembly, as one of the most enriched proteins in CENP\u2010A immunoprecipitates, suggesting an association between SET and CENP\u2010A. Here, we investigated this association and its functional significance. Reciprocal co\u2010immunoprecipitation assays showed that SET associates with both CENP\u2010A and HJURP. Immunofluorescence analysis revealed that SET localizes to centromeres and that SET depletion reduces centromeric CENP\u2010A enrichment. Live\u2010cell imaging further showed that SET depletion causes chromosome alignment defects and prolonged mitotic arrest. Together, these findings identify SET as a previously unrecognized regulator of the CENP\u2010A\u2010HJURP axis and suggest that SET supports proper CENP\u2010A localization at centromeres and faithful mitotic progression. This study expands our understanding of the regulatory network that maintains centromere identity.<\/jats:p>","DOI":"10.1002\/cbin.70221","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:01:43Z","timestamp":1790769703000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["SET Interacts With CENP\u2010A and HJURP to Ensure Proper Mitotic Progression in Human Cells"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6991-4640","authenticated-orcid":false,"given":"Ying","family":"Wang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, School of Life Sciences University of Science and Technology of China Hefei China"},{"name":"Hefei National Research Center for Interdisciplinary Sciences at the Microscale University of Science and Technology of China Hefei China"},{"name":"Anhui Key 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