{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:45:15Z","timestamp":1792147515427,"version":"4.4.0"},"reference-count":69,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Automated micronucleus (MN) scoring is increasingly proposed as a remedy for the subjectivity and labour burden of manual scoring yet reported performance has not been appraised systematically. This critical systematic review with quantitative synthesis follows PRISMA 2020. PubMed was searched for studies applying computer\u2010vision, machine\u2010learning, or deep\u2010learning methods to MN determination against expert or expert\u2010derived reference standards. Twenty\u2010four records were screened and eight (2020\u20132025) met eligibility, spanning fluorescence microscopy, brightfield\/Giemsa preparations, imaging flow cytometry, and whole\u2010slide imaging. Because no study reported a reconstructable 2\u2009\u00d7\u20092 table, bivariate diagnostic\u2010accuracy meta\u2010analysis was not possible; reported scalar metrics were instead logit\u2010transformed and combined by DerSimonian\u2013Laird random effects as explicitly descriptive summaries, with correlations combined on the Fisher\u2010z scale. Summarized recall was 0.924 (95% CI 0.845\u20130.965) and precision 0.874 (0.790\u20130.927), both with near\u2010total heterogeneity (I\n                    <jats:sup>2<\/jats:sup>\n                    \u2248 97%), whereas agreement between automated and reference counts was high and homogeneous (Pearson r\u2009=\u20090.920, 0.911\u20130.929; I\n                    <jats:sup>2<\/jats:sup>\n                    = 0%). Metric definition was the dominant moderator of heterogeneity; residual variation in precision tracked imaging modality rather than network architecture. Although there are bias regarding choice of staining or systems used for analysis as discussed here, current evidence supports a future of automated MN frequency estimation for regulatory and biomonitoring endpoints; but not object\u2010level equivalence with expert scoring.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70225","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:35:58Z","timestamp":1791380158000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["AI\u2010Assisted Micronucleus Detection: A Critical Systematic Review With Quantitative Synthesis"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4104-9209","authenticated-orcid":false,"given":"Somnath","family":"Paul","sequence":"first","affiliation":[{"name":"Department of Pathology and Immunology, School of Medicine Washington University St Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.30699\/IJP.2023.1989457.3062"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmpo.ijmpo_26_16"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.6168"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25432"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-016-9950-4"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02195"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.820.13.5"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-529-3_10"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geq.075"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgl177"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/XEB.0000000000000061"},{"key":"e_1_2_12_13_1","volume-title":"YOLO\u2010MN: High\u2010Throughput Cell Micronucleus Detection Based on Prior Knowledge and Frequency Domain Perception","author":"Cao L.","year":"2026"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pdig.0000022"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2013.01.030"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-02857-3"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2023-078378"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cct.2015.09.002"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-025-03982-3"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.101579"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/ftox.2023.1171960"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.77"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1383-5718(02)00249-8"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2024.108511"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2010.05.011"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10653-012-9469-8"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553002.2019.1625462"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2015.015"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature23470"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24698-1"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-07796-4"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3348\/kjr.2019.0025"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-83267-w"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2589-7500(19)30123-2"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-02856-4"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0360-8"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature23449"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07619-7"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02151-z"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1148\/ryai.2020200029"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.m689"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-025-01516-3"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-06-0248"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2016.04.002"},{"key":"e_1_2_12_45_1","unstructured":"OECD.2014.Test No. 487: In Vitro Mammalian Cell Micronucleus Test. Retrieved fromhttps:\/\/www.oecd.org\/en\/publications\/test-no-487-in-vitro-mammalian-cell-micronucleus-test_9789264224438-en.html."},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n71"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmpb.2023.107447"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2016.411"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-013-9652-0"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.70142"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/jes.2012.91"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2026.2665801"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41540-021-00179-5"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrsm.1348"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07936-4"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geaf026"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.93101s357"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.53"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-017-0496-y"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v036.i03"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-03141-0"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12911-022-01875-w"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-155-8-201110180-00009"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics15081032"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07441-w"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41021-024-00305-9"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-026-41458-7"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14493"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-73428-2"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.6263"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.70225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.70225","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.70225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:53:07Z","timestamp":1792144387000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.70225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":69,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/cbin.70225"],"URL":"https:\/\/doi.org\/10.1002\/cbin.70225","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70225"}}