{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:40:30Z","timestamp":1792417230199,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    HSPD1 is an important chaperone protein whose primary function is to assist the correct folding of mitochondrial proteins and its co\u2010chaperone. It exerts multiple biological effects, including participation in protein homeostasis maintenance, stress resistance, and cellular protection. However, whether\n                    <jats:italic>Hspd1<\/jats:italic>\n                    modulates the proliferation and differentiation of C2C12 cells remains unclear. In this study, we observed that overexpression or knockdown of the\n                    <jats:italic>Hspd1<\/jats:italic>\n                    gene increased or decreased C2C12 cell proliferation, respectively, whereas differentiation exhibited the opposite trend. Furthermore, co\u2010immunoprecipitation coupled with mass spectrometry identified several putative HSPD1\u2010interacting partners. Functional assays revealed that altering\n                    <jats:italic>Hspd1<\/jats:italic>\n                    expression correlated with changes in the expression of mitochondrial morphology\u2010related genes, transcripts of components of the tricarboxylic acid cycle and electron transport chain, as well as intracellular ATP levels. Although these results are consistent with the hypothesis that HSPD1\u2010associated mitochondrial alterations may contribute to myoblast behavior, they do not exclude other potential mechanisms. Collectively, this study provides correlative evidence linking\n                    <jats:italic>Hspd1<\/jats:italic>\n                    to mitochondrial regulation in C2C12 cells and underscores the necessity for further mechanistic dissection, including rescue experiments to establish causality.\n                  <\/jats:p>","DOI":"10.1002\/cbin.70228","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:46:03Z","timestamp":1792413963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HSPD1 Regulates C2C12 Cell Proliferation and Differentiation in Association With Mitochondrial Function Changes"],"prefix":"10.1002","volume":"50","author":[{"given":"Yuhui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijing","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8287-5109","authenticated-orcid":false,"given":"Wentian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingjun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingzhu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6569-8401","authenticated-orcid":false,"given":"Weiwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Life Science and Agroforestry Qiqihar University Qiqihar Heilongjiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600910RR"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-017-9467-7"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/337620a0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020449"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.567378"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.08.014"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2020.154257"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13619-021-00093-5"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.12891"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.161"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-008-0068-7"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2018.4608"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03510-6"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.9b00086"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7120224"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11030835"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2020.04.009"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90005-6"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-3036-5_11"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2017.299"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1996.0003"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488278"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414-023-02971-w"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.595"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610647104"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.102978"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.23812\/20-60-A"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16569"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.4149\/av_2018_104"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-024-00978-0"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051830"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.11.011"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-42944-y"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.70228","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.70228","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.70228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:46:04Z","timestamp":1792413964000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.70228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,19]]},"references-count":33,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/cbin.70228"],"URL":"https:\/\/doi.org\/10.1002\/cbin.70228","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,19]]},"assertion":[{"value":"2026-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70228"}}