{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T07:05:10Z","timestamp":1771225510096,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cathet Cardio Intervent"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Extracorporeal membrane oxygenation (ECMO) is an effective treatment for cardiopulmonary failure. However, it is associated with severe complications, including cerebral injury, which contribute to elevated mortality and disability rates. The administration of antibiotics may lead to gut microbiome dysbiosis among critically ill patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aims to investigate the association between antibiotic\u2010induced gut microbiome dysbiosis and cerebral injury during ECMO treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The compositional changes in the gut microbiome induced by antibiotic (ABX) treatment were analyzed using microbiome analysis techniques. ECMO treatment models were established by using rat. Brain tissue pathology was assessed using H&amp;E and Nissl staining. Serum concentrations of S100\u03b2 and NSE were quantified using ELISA. Pro\u2010inflammatory factors in the brain and serum were analyzed, and microglial activation was evaluated via immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Gut microbiome dysbiosis induced by ABX treatment. Compared to the sham group, significant cerebral injury was observed in both the ECMO and ECMO\u2010ABX groups. The expression levels of S100\u03b2 and NSE were significantly elevated in the ECMO\u2010ABX group. Additionally, parameters of microglial activation, such as cell body area, total branch length, mean length of branches, and number of branch points, were significantly increased in the ECMO\u2010ABX group compared to the ECMO group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrates that cerebral injury occurs during ECMO treatment, and antibiotic\u2010induced gut microbiome dysbiosis may exacerbate this cerebral injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ccd.31589","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:35:17Z","timestamp":1747190117000},"page":"590-599","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibiotic\u2010Induced Gut Microbiome Dysbiosis Aggravates Cerebral Injury During Extracorporeal Membrane Oxygenation"],"prefix":"10.1002","volume":"106","author":[{"given":"Kangmei","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Neurology Lanzhou University Second Hospital Lanzhou China"},{"name":"Frontier Science Center of Rare Isotopes Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Neurology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilin","family":"Wei","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Lanzhou University Second Hospital Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongnan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Lanzhou University Second Hospital Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1620-7351","authenticated-orcid":false,"given":"Zhaoming","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Neurology Lanzhou University Second Hospital Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-014-0565-2"},{"key":"e_1_2_11_3_1","unstructured":"ELSO Live Registry Dashboard. 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