{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T05:16:21Z","timestamp":1775106981782,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cathet Cardio Intervent"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Coronary artery disease (CAD) remains a leading global cause of mortality, with percutaneous coronary intervention (PCI) being a primary treatment. However, in\u2010stent restenosis (ISR) affects 15%\u221230% of cases, driven by vascular injury, inflammation, and smooth muscle cell proliferation. While drug\u2010eluting stents reduce ISR rates, late complications persist. Low\u2010level laser therapy (LLLT) has emerged as a potential adjunctive treatment, modulating inflammation and cell behavior, but its effects on endothelial cells miRNA regulation remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study investigated LLLT's impact on human umbilical vein endothelial cell (HUVEC) migration, proliferation, and miR\u2010143 expression, a key regulator and potential biomarker in vascular restenosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>HUVECs were cultured and exposed to 532 and 633\u2009nm lasers at varying durations (10\u2013200\u2009s). Cell migration was assessed via scratch wound assays, with imaging at 1, 5\u2010, 12\u2010, 24\u2010, and 48\u2010h post\u2010irradiation. Proliferation and viability were quantified using image analysis (MATLAB). For gene expression, irradiated cells were incubated for 24\u2009h, followed by RNA extraction, cDNA synthesis, and qRT\u2010PCR to measure miR\u2010143 levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The 532\u2009nm laser inhibited HUVEC migration most effectively at 10\u2009s, minimizing cytotoxicity. In contrast, the 633\u2009nm laser required longer exposure (200\u2009s) for comparable inhibition but induced delayed miR\u2010143 upregulation after 24\u2009h, suggesting epigenetic modulation. Both wavelengths influenced cell behavior in a time\u2010 and dose\u2010dependent manner, with 532\u2009nm offering rapid effects and 633\u2009nm potentially exerting longer\u2010term genetic regulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LLLT at specific wavelengths differentially modulates endothelial cell migration and miR\u2010143 expression, highlighting its potential as a non\u2010invasive strategy to mitigate restenosis. The 532\u2009nm laser provides immediate anti\u2010migratory effects, while 633\u2009nm may offer sustained benefits via miRNA regulation. Further research should optimize parameters and validate these findings in vivo to advance LLLT as a complementary therapy for vascular interventions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ccd.70483","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T05:56:19Z","timestamp":1770616579000},"page":"1514-1524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low\u2010Power Lasers Therapy Induced Mir\u2010143 Upregulation: A Novel Biomarker and Therapeutic Strategy for Restenosis"],"prefix":"10.1002","volume":"107","author":[{"given":"Atiyeh","family":"Asadi","sequence":"first","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9223-5565","authenticated-orcid":false,"given":"Amir Ghafari","family":"Jolfayi","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Center Rajaie Cardiovascular Institute Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6646-6274","authenticated-orcid":false,"given":"Rouhollah","family":"Karimzadeh","sequence":"additional","affiliation":[{"name":"Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammadreza","family":"Babaei","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Center Rajaie Cardiovascular Institute Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maedeh","family":"Arabian","sequence":"additional","affiliation":[{"name":"Cardiogenetic Research Center Rajaie Cardiovascular Institute Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2991\/jegh.k.201217.001"},{"key":"e_1_2_10_3_1","unstructured":"M.Ahmad P.Mehta A.Reddivari andS.Mungee. 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