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Their applications include the reduction of cyclic imines and the reductive amination of prochiral ketones. In this study, twenty\u2010nine novel IREDs were revealed through genome mining. Imine reductase activities were screened at pH\u20057 and 9 and in presence of either NADPH or NADH; some IREDs showed good activities at both pHs and were able to accept both cofactors. IREDs with Asn and Glu at the key 187 residue showed preference for NADH. IREDs were also screened against a series of dihydroisoquinolines to synthesise tetrahydroisoquinolines (THIQs), bioactive alkaloids with a wide range of therapeutic properties. Selected IREDs showed high stereoselectivity, as well high THIQ yields (&gt;90\u2009%) when coupled to a glucose\u20106\u2010phosphate dehydrogenase for NADPH cofactor recycling.<\/jats:p>","DOI":"10.1002\/cctc.202201126","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T06:07:08Z","timestamp":1670998028000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Discovery of Imine Reductases and their Utilisation for the Synthesis of Tetrahydroisoquinolines"],"prefix":"10.1002","volume":"15","author":[{"given":"Max","family":"C\u00e1rdenas\u2010Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering University College London  Gower Street, Bernard Katz Building London WC1E 6BT UK"},{"name":"School of Biosciences University of Kent K  ent CT2 7NJ 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