{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:53:56Z","timestamp":1791460436294,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chronic Diseases and Translational Medicine"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cardiovascular disease remains a major public health burden. This study aimed to investigate the effect of the methylenetetrahydrofolate reductase (MTHFR) C677T genotype on folic acid treatment response and cardiovascular endothelial function in patients with hyperhomocysteinemia (HHcy).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We retrospectively enrolled 344 patients with HHcy genotyped for MTHFR C677T (CC,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009141; CT,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009169; and TT,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200934). Plasma homocysteine (Hcy) concentrations and vascular function indicators were measured at baseline and 2, 4, and 12 months. Repeated\u2010measures one\u2010way analysis of variance, Kaplan\u2013Meier analysis, and multivariate logistic regression were applied.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After treatment, the Hcy concentration and pulse wave velocity decreased over time in all genotype groups, whereas the ankle\u2010brachial index increased. Significant main effects of time were observed for Hcy concentration (\n                      <jats:italic>F<\/jats:italic>\n                      \u2009=\u2009173.52,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), pulse wave velocity (\n                      <jats:italic>F<\/jats:italic>\n                      \u2009=\u200952.84,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), and ankle\u2010brachial index (\n                      <jats:italic>F<\/jats:italic>\n                      \u2009=\u200994.17,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). At each time point, patients with CC showed better values than patients with CT and TT for all parameters (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) except for intima\u2010media thickness in patients with TT, which showed no significant changes (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Patients with CC also had the highest Hcy concentration target achievement rate (81.6%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01 vs. CT and TT). Patients with the CC genotype had the highest cumulative Hcy concentration target achievement rate (81.6%), which was higher than that of patients with the CT and TT genotypes (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Multivariate logistic regression showed that the CT (OR\u2009=\u20090.320, 95% CI: 0.179\u20130.572) and TT (OR\u2009=\u20090.080, 95% CI: 0.019\u20130.326) genotypes were independent negative predictors of target achievement, whereas higher folate metabolism capacity was a positive predictor.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MTHFR C677T polymorphism significantly affects folic acid efficacy and endothelial improvement. Patients with the TT genotype had lower therapeutic efficacy of folic acid therapy. Genotyping may help estimate treatment responses and support individualized follow\u2010up.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cdt3.70068","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:14:56Z","timestamp":1791458096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effect of the MTHFR C677T Genotype Polymorphism on the Efficacy of Folic Acid Treatment for Hyperhomocysteinemia and Cardiovascular Endothelial Function"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0423-8668","authenticated-orcid":false,"given":"Xiaohui","family":"Dou","sequence":"first","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liheng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahui","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxia","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Health Management Zhuhai People's Hospital Zhuhai Guangdong 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