{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:50:06Z","timestamp":1791600606098,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":128,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007795","name":"Stowers Institute for Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Congenital Anomalies"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Craniofacial development requires precise coordination of epithelial patterning and morphogenesis. However, the molecular mechanisms governing olfactory epithelium development remain incompletely understood. Retinoic acid (RA) signaling and\n                    <jats:italic>Gata3<\/jats:italic>\n                    have each been independently implicated in craniofacial morphogenesis, particularly in the formation of the primitive choanae, which constitute the opening between the nasal cavity and the oral cavity. Here, we generated a tamoxifen\u2010inducible, genetically controlled compound mutant mouse model to simultaneously disrupt\n                    <jats:italic>Rdh10<\/jats:italic>\n                    , a rate\u2010limiting enzyme for RA signaling, and\n                    <jats:italic>Gata3<\/jats:italic>\n                    during early craniofacial development. We show that while deletion of\n                    <jats:italic>Rdh10<\/jats:italic>\n                    following tamoxifen administration at E8.5 does not result in obvious craniofacial abnormalities, combined loss of\n                    <jats:italic>Rdh10<\/jats:italic>\n                    and\n                    <jats:italic>Gata3<\/jats:italic>\n                    leads to fully penetrant bilateral choanal atresia and severe defects in olfactory epithelium morphogenesis. Immunohistochemical analyses revealed a marked reduction in PAX6 and SIX1 positive cells and a concomitant expansion of SOX2 positive cells in compound mutant embryos. These results demonstrate that RA\u2013Gata3 signaling cooperatively regulates olfactory epithelium development by controlling the balance between progenitor maintenance and lineage specification. We propose that the RA\u2013Gata3 signaling pathway orchestrates a transcriptional network involving\n                    <jats:italic>Pax6<\/jats:italic>\n                    ,\n                    <jats:italic>Six1<\/jats:italic>\n                    , and\n                    <jats:italic>Sox2<\/jats:italic>\n                    to ensure proper epithelial patterning, branching morphogenesis, and choanae formation during early craniofacial development.\n                  <\/jats:p>","DOI":"10.1002\/cga.70060","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T11:39:41Z","timestamp":1778326781000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Role of Retinoid and Gata3 Signaling in Olfactory Epithelium Development"],"prefix":"10.1002","volume":"66","author":[{"given":"Ayaka","family":"Matsushita","sequence":"first","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics The University of Osaka Graduate School of Dentistry  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Tsujimoto","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics The University of Osaka Graduate School of Dentistry  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nie","family":"Xiuping","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics The University of Osaka Graduate School of Dentistry  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1347-9044","authenticated-orcid":false,"given":"Maiko","family":"Omi\u2010Sugihara","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics The University of Osaka Graduate School of Dentistry  Osaka 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Graduate School of Dentistry  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Koga","sequence":"additional","affiliation":[{"name":"Laboratory for Innate Immune Systems, Department of Microbiology and Immunology, Graduate School of Medicine The University of Osaka  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5303-4763","authenticated-orcid":false,"given":"Toshihiro","family":"Inubushi","sequence":"additional","affiliation":[{"name":"Department of Orthodontics and Dentofacial Orthopedics The University of Osaka Graduate School of Dentistry  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa L.","family":"Sandell","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases University of Louisville  Louisville Kentucky 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