{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:27:12Z","timestamp":1778653632957,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2007,3,20]],"date-time":"2007-03-20T00:00:00Z","timestamp":1174348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2007,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid and quantitative method for solid\u2010phase methyl esterification of carboxy groups of various sialylated oligosaccharides has been established. The method employed a triazene derivative, 3\u2010methyl\u20101\u2010<jats:italic>p\u2010<\/jats:italic>tolyltriazene, for facile derivatization of oligosaccharides immobilized onto general solid supports such as Affi\u2010Gel\u2005Hz and gold colloidal nanoparticles in a multiwell plate. The workflow protocol was optimized for the solid\u2010phase processing of captured sialylated\/unsialylated oligosaccharides separated from crude sample mixtures by chemical ligation. From tryptic and\/or PNGase F\u2010digest mixtures of glycoproteins, purification by chemoselective immobilization, esterification and recovery were achieved in the same well of the filter plate within three hours when used in conjunction with \u201cglycoblotting technology\u201d (S.\u2010I. Nishimura, K. Niikura, M. Kurogochi, T. Matsushita, M. Fumoto, H. Hinou, R. Kamitani, H. Nakagawa, K. Deguchi, N. Miura, K. Monde, H. Kondo, High\u2010throughput protein glycomics: Combined use of chemoselective glycoblotting and MALDI\u2010TOF\/TOF mass spectrometry: <jats:italic>Angew. Chem.<\/jats:italic> <jats:bold>2005<\/jats:bold>, <jats:italic>117<\/jats:italic>, 93\u201398; <jats:italic>Angew. Chem. Int. Ed<\/jats:italic>. <jats:bold>2005<\/jats:bold>, <jats:italic>44<\/jats:italic>, 91\u201396). The recovered materials were directly applicable to subsequent characterization by mass spectrometric techniques such as MALDI\u2010TOF for large\u2010scale glycomics of both neutral and sialylated oligosaccharides. On\u2010bead\/on\u2010gold nanoparticle derivatization of glycans containing sialic acids allowed rapid and quantitative glycoform profiling by MALDI\u2010TOF MS with reflector and positive ion mode. In addition to its simplicity and speed, the method eliminates the use of unfavorable halogenated solvents such as chloroform and dichloromethane or volatile solvents such as diethyl ether and hexane, resulting in a practical and green chemical method for automated robotic adaptation.<\/jats:p>","DOI":"10.1002\/chem.200601872","type":"journal-article","created":{"date-parts":[[2007,3,20]],"date-time":"2007-03-20T07:32:39Z","timestamp":1174375959000},"page":"4797-4804","source":"Crossref","is-referenced-by-count":113,"title":["Rapid and Simple Solid\u2010Phase Esterification of Sialic Acid Residues for Quantitative Glycomics by Mass 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