{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T23:46:46Z","timestamp":1788652006527,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"28","license":[{"start":{"date-parts":[[2007,7,4]],"date-time":"2007-07-04T00:00:00Z","timestamp":1183507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2007,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This work demonstrates the use of photocleavable cholesterol derivatives to create supported bilayer lipid membrane arrays on silica. The photocleavable cholesteryl tether is attached to the surface by using the reaction of an amine\u2010functionalized self\u2010assembled monolayer (SAM) and the <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide\u2010based reagent <jats:bold>9<\/jats:bold>. The resultant SAM contains an <jats:italic>ortho<\/jats:italic>\u2010nitrobenzyl residue that can be cleaved by photolysis by using soft (365\u2005nm) UV light regenerating the original amine surface, and which can be patterned using a mask. The photoreaction yield was \u224875\u2009% which was significantly higher than previously found for related <jats:italic>ortho<\/jats:italic>\u2010nitrobenzyl photochemistry on gold substrates. The SAMs were characterized by means of contact angle measurements, ellipsometry and X\u2010ray photoelectron spectroscopy. Patterned surfaces were characterized with SEM and AFM. After immersing the patterned surface into a solution containing small unilamellar vesicles of egg phosphatidylcholine (PC), supported lipid membranes were formed comprised of lipid bilayer over the amine functionalized \u201chydrophilic\u201d regions and lipid monolayer over the cholesteryl \u201chydrophobic\u201d regions. This was confirmed by fluorescence microscopy and AFM. FRAP studies yielded a lateral diffusion coefficient for the probe molecule of 0.14\u00b10.05\u2005\u03bcm<jats:sup>2<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup> in the bilayer regions and \u22480.01\u2005\u03bcm<jats:sup>2<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup> in the monolayer regions. This order of magnitude difference in diffusion coefficients effectively serves to isolate the bilayer regions from one another, thus creating a bilayer array.<\/jats:p>","DOI":"10.1002\/chem.200700521","type":"journal-article","created":{"date-parts":[[2007,7,4]],"date-time":"2007-07-04T10:22:30Z","timestamp":1183544550000},"page":"7957-7964","source":"Crossref","is-referenced-by-count":36,"title":["Supported Bilayer Lipid Membrane Arrays on Photopatterned Self\u2010Assembled Monolayers"],"prefix":"10.1002","volume":"13","author":[{"given":"Xiaojun","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Singh\u2005N.\u2005D.","family":"Pradeep","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Critchley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khizar","family":"Sheikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard\u2005J.","family":"Bushby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen\u2005D.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,9,19]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.12.3561"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/194979a0"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.02.033"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/la970600k"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/la0342060"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5245.43"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-0352(00)00017-9"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(99)01412-2"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200390048"},{"key":"e_1_2_6_9_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200390080"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04164"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/la034197v"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1557\/mrs2005.54"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/la960805d"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/42432"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/35093038"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/la060121y"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.675"},{"key":"e_1_2_6_18_2","first-page":"606","volume":"5","author":"Groves J. 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