{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:56:02Z","timestamp":1791150962955,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2010,3,22]],"date-time":"2010-03-22T00:00:00Z","timestamp":1269216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2010,4,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An organometallic cyclometalated platinum(II) complex, [Pt(L<jats:sup>3<\/jats:sup>)Cl][PF<jats:sub>6<\/jats:sub>], has been synthesised from a specially designed cyclometalating ligand, HL<jats:sup>3<\/jats:sup> (triphenyl{5\u2010[3\u2010(6\u2010phenylpyridin\u20102\u2010yl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20101\u2010yl]pentyl}phosphonium chloride), that contains a pendant carbon chain carrying a terminal cationic triphenylphosphonium moiety. Aside from its room temperature single\u2010photon luminescent properties in solution, [Pt(L<jats:sup>3<\/jats:sup>)Cl]<jats:sup>+<\/jats:sup> can also produce two\u2010photon\u2010induced luminescence at room temperature upon excitation at 700\u2005nm from a mode\u2010locked Ti:sapphire laser. Its two\u2010photon absorption cross\u2010section in DMF at room temperature was measured to be 28.0\u00d710<jats:sup>\u221250<\/jats:sup>\u2005cm<jats:sup>4<\/jats:sup>\u2009s\u2009photon<jats:sup>\u22121<\/jats:sup>. [Pt(L<jats:sup>3<\/jats:sup>)Cl]<jats:sup>+<\/jats:sup> is able to selectively stain the cell nucleolus. This has been demonstrated by two\u2010photon confocal imaging of live and methanol\u2010fixed HeLa (human cervical carcinoma) and 3T3 (mouse skin fibroblasts) cells. This organelle specificity is likely to be related to its special affinity for proteins within cell nucleoli. As a result of such protein affinity, [Pt(L<jats:sup>3<\/jats:sup>)Cl]<jats:sup>+<\/jats:sup> is an efficient RNA transcription inhibitor and shows rather profound cytotoxicity. On the other hand, the organelle\u2010specific labelling and two\u2010photon\u2010induced luminescent properties of [Pt(L<jats:sup>3<\/jats:sup>)Cl]<jats:sup>+<\/jats:sup> renders it a useful nuclear dye for the 3\u2010dimensional reconstruction of optical sections of thick tissues, for example, mouse ileum tissues, by multiphoton confocal microscopy.<\/jats:p>","DOI":"10.1002\/chem.200902919","type":"journal-article","created":{"date-parts":[[2010,3,22]],"date-time":"2010-03-22T11:44:01Z","timestamp":1269258241000},"page":"3942-3950","source":"Crossref","is-referenced-by-count":69,"title":["A Triphenylphosphonium\u2010Functionalised Cyclometalated Platinum(II) Complex as a Nucleolus\u2010Specific Two\u2010Photon Molecular Dye"],"prefix":"10.1002","volume":"16","author":[{"given":"Chi\u2010Kin","family":"Koo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leo\u2005K.\u2010Y.","family":"So","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka\u2010Leung","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Man","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Wah","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael\u2005H.\u2010W.","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwok\u2010Wai","family":"Cheah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chopen\u2005Chan\u2010Wut","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai\u2010Ming","family":"Kwok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,3,26]]},"reference":[{"key":"e_1_2_6_1_2","unstructured":"\u00a0"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/b517519d"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200501390"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja056303g"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.12.005"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200701357"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/b718993a"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200705155"},{"key":"e_1_2_6_8_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705155"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/b804434c"},{"key":"e_1_2_6_10_2","unstructured":"\u00a0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr9804341"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0677564"},{"key":"e_1_2_6_12_3","doi-asserted-by":"publisher","DOI":"10.1021\/ja9025165"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/b900290a"},{"key":"e_1_2_6_14_2","unstructured":"\u00a0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1039\/b800939b"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/om800212t"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200705155"},{"key":"e_1_2_6_17_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705155"},{"key":"e_1_2_6_18_2","first-page":"709","volume":"48","author":"Lau J. 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