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While the good spectral overlap between the emission of BODIPY and the absorption of TMR was one favorable factor, another feature of this FRET system was the rigid and short biphenyl spacer that favored efficient through\u2010bond energy transfer. More importantly, in this system, the 2\u2032\u2010carboxyl group of the rhodamine unit was preserved for the further modifications, which was as convenient as those carbonyl groups on the original rhodamines without connection to donors. For this reason, BRP is clearly differentiated from the previous ratiometric sensors based on donor rhodamine systems. To illustrate its value as a versatile platform, we introduced typical Hg<jats:sup>2+<\/jats:sup> receptors into BRP, through convenient one\u2010pot reactions on the 2\u2032\u2010carboxyl group, and successfully developed two ratiometric sensors, BRP\u20101 and BRP\u20102, with different spirocyclic receptors that recognized Hg<jats:sup>2+<\/jats:sup> on different reaction mechanisms. Upon excitation at a single wavelength (488\u2005nm), at which only BODIPY absorbed, both of the FRET sensors exhibited clear Hg<jats:sup>2+<\/jats:sup>\u2010induced changes in the intensity ratio of the two strong emission bands of BODIPY and rhodamine. It should be noted that these ratiometric Hg<jats:sup>2+<\/jats:sup> sensors exhibited excellent sensitivity and selectivity Hg<jats:sup>2+<\/jats:sup>, as well as pH insensitivity, which was similar to the corresponding \u2018turn\u2010on\u2019 rhodamine sensors. While both ratiometric probes were applicable for Hg<jats:sup>2+<\/jats:sup> imaging in living cells, BRP\u20101 exhibited higher sensitivity and faster responses than BRP\u20102. Our investigation indicated that on a versatile platform, such as BRP, a large number of highly efficient ratiometric sensors for transition\u2010metal ions could be conveniently developed.<\/jats:p>","DOI":"10.1002\/chem.201002498","type":"journal-article","created":{"date-parts":[[2011,2,10]],"date-time":"2011-02-10T18:47:11Z","timestamp":1297363631000},"page":"3179-3191","source":"Crossref","is-referenced-by-count":141,"title":["Convenient and Efficient FRET Platform Featuring a Rigid Biphenyl Spacer between Rhodamine and BODIPY: Transformation of \u2018Turn\u2010On\u2019 Sensors into Ratiometric Ones with Dual 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