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Since <jats:bold>1<\/jats:bold> was extremely sensitive to oxygen, a protective isolation and handling protocol was developed, by utilizing the free radical scavenger 4\u2010ethoxyphenol. The structure of <jats:bold>1<\/jats:bold> was determined by HRMS, 1D and 2D NMR spectroscopy and chemical derivatization to acetonides and Mosher esters to finally establish the absolute configuration. Methionine and acetate were identified as building blocks in the biosynthesis of <jats:bold>1<\/jats:bold> by feeding experiments with differently <jats:sup>13<\/jats:sup>C\u2010labeled precursors. The antimicrobial activity of <jats:bold>1<\/jats:bold> was determined in a broad screening revealing <jats:bold>1<\/jats:bold> to inhibit several Gram\u2010negative bacteria.<\/jats:p>","DOI":"10.1002\/chem.201003677","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T08:34:12Z","timestamp":1306398852000},"page":"7875-7881","source":"Crossref","is-referenced-by-count":17,"title":["Roimatacene: An Antibiotic against Gram\u2010Negative Bacteria Isolated from <i>Cystobacter ferrugineus<\/i> Cb G35 (Myxobacteria)"],"prefix":"10.1002","volume":"17","author":[{"given":"Wiebke","family":"Zander","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Gerth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathrin I.","family":"Mohr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Kessler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,5,26]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.07.004"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70143-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201001279"},{"key":"e_1_2_6_4_2","first-page":"220","volume":"12","author":"Wenzel S. 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