{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:33:10Z","timestamp":1778668390691,"version":"3.51.4"},"reference-count":76,"publisher":"Wiley","issue":"31","license":[{"start":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T00:00:00Z","timestamp":1403568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2014,7,28]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A multitechnique approach has been applied in order to identify the thermodynamic and kinetic parameters related to the regioselective hydrolysis of human serum albumin (HSA) promoted by the Wells\u2013Dawson polyoxometalate (POM), K<jats:sub>15<\/jats:sub>H[Zr(\u03b1<jats:sub>2<\/jats:sub>\u2010P<jats:sub>2<\/jats:sub>W<jats:sub>17<\/jats:sub>O<jats:sub>61<\/jats:sub>)<jats:sub>2<\/jats:sub>]. Isothermal titration calorimetry (ITC) studies indicate that up to four POM molecules interact with HSA. While the first interaction site is characterized by a 1:1 binding and an affinity constant of 2\u00d710<jats:sup>8<\/jats:sup>\u2009<jats:sc>M<\/jats:sc><jats:sup>\u22121<\/jats:sup>, the three remaining sites are characterized by a lower global affinity constant of 7\u00d710<jats:sup>5<\/jats:sup>\u2009<jats:sc>M<\/jats:sc><jats:sup>\u22121<\/jats:sup>. The higher affinity constant at the first site is in accordance with a high quenching constant of 2.2\u00d710<jats:sup>8<\/jats:sup>\u2009<jats:sc>M<\/jats:sc><jats:sup>\u22121<\/jats:sup> obtained for fluorescence quenching of the Trp214 residue located in the only positively charged cleft of HSA, in the presence of K<jats:sub>15<\/jats:sub>H[Zr(\u03b1<jats:sub>2<\/jats:sub>\u2010P<jats:sub>2<\/jats:sub>W<jats:sub>17<\/jats:sub>O<jats:sub>61<\/jats:sub>)<jats:sub>2<\/jats:sub>]. In addition, Eu<jats:sup>III<\/jats:sup> luminescence experiments with an Eu<jats:sup>III<\/jats:sup>\u2010substituted POM analogue have shown the replacement of water molecules in the first coordination sphere of Eu<jats:sup>III<\/jats:sup> due to binding of the metal ion to amino acid side chain residues of HSA. All three interaction studies are in accordance with a stronger POM dominated binding at the positive cleft on the one hand, and interaction mainly governed by metal anchoring at the three remaining positions, on the other hand. Hydrolysis experiments in the presence of K<jats:sub>15<\/jats:sub>H[Zr(\u03b1<jats:sub>2<\/jats:sub>\u2010P<jats:sub>2<\/jats:sub>W<jats:sub>17<\/jats:sub>O<jats:sub>61<\/jats:sub>)<jats:sub>2<\/jats:sub>] have demonstrated regioselective cleavage of HSA at the Arg114\uf8ffLeu115, Ala257\uf8ffAsp258, Lys313\uf8ffAsp314 or Cys392\uf8ffGlu393 peptide bonds. This is in agreement with the interaction studies as the Arg114\uf8ffLeu115 peptide bond is located in the positive cleft of HSA and the three remaining peptide bonds are each located near an upstream acidic residue, which can be expected to coordinate to the metal ion. A detailed kinetic study has evidenced the formation of additional fragments upon prolonged reaction times. Edman degradation of the additional reaction products has shown that these fragments result from further hydrolysis at the initially observed cleavage positions, indicating a fixed selectivity for K<jats:sub>15<\/jats:sub>H[Zr(\u03b1<jats:sub>2<\/jats:sub>\u2010P<jats:sub>2<\/jats:sub>W<jats:sub>17<\/jats:sub>O<jats:sub>61<\/jats:sub>)<jats:sub>2<\/jats:sub>].<\/jats:p>","DOI":"10.1002\/chem.201402683","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T10:11:13Z","timestamp":1403604673000},"page":"9567-9577","source":"Crossref","is-referenced-by-count":54,"title":["Molecular Origin of the Hydrolytic Activity and Fixed Regioselectivity of a Zr<sup>IV<\/sup>\u2010Substituted Polyoxotungstate as Artificial Protease"],"prefix":"10.1002","volume":"20","author":[{"given":"Karen","family":"Stroobants","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Goovaerts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Absillis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Bruylants","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Moelants","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Proost","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatjana 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