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AIM\u20101 is one of only a few MBLs from the B3 subgroup that is encoded on a mobile genetic element in a major human pathogen. Here, its mechanism of action was characterised with a combination of spectroscopic and kinetic techniques and compared to that of other MBLs. Unlike other MBLs it appears that AIM\u20101 has two avenues available for the turnover of the substrate nitrocefin, distinguished by the identity of the rate\u2010limiting step. This observation may be relevant with respect to inhibitor design for this group of enzymes as it demonstrates that at least some MBLs are very flexible in terms of interactions with substrates and possibly inhibitors.<\/jats:p>","DOI":"10.1002\/chem.201602762","type":"journal-article","created":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T03:26:38Z","timestamp":1477365998000},"page":"17704-17714","source":"Crossref","is-referenced-by-count":34,"title":["AIM\u20101: An Antibiotic\u2010Degrading Metallohydrolase That Displays Mechanistic Flexibility"],"prefix":"10.1002","volume":"22","author":[{"given":"Christopher","family":"Selleck","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  St. Lucia Queensland 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Larrabee","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Middlebury College  Middlebury Vermont 05753 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Harmer","sequence":"additional","affiliation":[{"name":"Centre for Advanced Imaging The University of Queensland  St. Lucia Queensland 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke W.","family":"Guddat","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  St. Lucia Queensland 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nata\u0161a","family":"Miti\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry Maynooth University  Maynooth, Co. 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