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The implementation of multivalency in enzyme inhibition is rather recent, being exemplified by few serendipitous discoveries, and hitherto relying on the random exploration of new multivalent structures as potential enzyme inhibitors. Here, a straightforward and versatile method is reported that enables the construction of multivalent systems for the inhibition of carbonic anhydrases (CA), widespread enzymes that catalyze a fundamental biochemical reaction. Oxime and hydrazone click\u2010type bioconjugation techniques were successfully used for the preparation of tetravalent peptide conjugates tethered with sulfonamide CA inhibitors. The enzyme inhibition assays show that multivalent effects were present with these novel compounds, but also reveal various structural effects provided by the scaffolds. The versatility of this approach may facilitate the exploration of structure\u2013activity relationships for other types of enzyme inhibitors.<\/jats:p>","DOI":"10.1002\/chem.201700241","type":"journal-article","created":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T06:22:42Z","timestamp":1487744562000},"page":"6788-6794","source":"Crossref","is-referenced-by-count":23,"title":["Effective Access to Multivalent Inhibitors of Carbonic Anhydrases Promoted by Peptide Bioconjugation"],"prefix":"10.1002","volume":"23","author":[{"given":"Nasreddine","family":"Kanfar","sequence":"first","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, UMR 5247 CNRS, ENSCM Universit\u00e9 de Montpellier  8 rue de l'Ecole Normale 34296 Montpellier Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammet","family":"Tanc","sequence":"additional","affiliation":[{"name":"Neurofarba Department; Section of Pharmaceutical and Nutriceutical Sciences Universit\u00e0 degli Studi di Firenze  Via Ugo Schiff 6 50019 Sesto Fiorentino Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Dumy","sequence":"additional","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, UMR 5247 CNRS, ENSCM Universit\u00e9 de Montpellier  8 rue de l'Ecole Normale 34296 Montpellier Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"Neurofarba Department; Section of Pharmaceutical and Nutriceutical Sciences Universit\u00e0 degli Studi di Firenze  Via Ugo Schiff 6 50019 Sesto Fiorentino Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6080-3345","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Ulrich","sequence":"additional","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, UMR 5247 CNRS, ENSCM Universit\u00e9 de Montpellier  8 rue de l'Ecole Normale 34296 Montpellier Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3197-3414","authenticated-orcid":false,"given":"Jean\u2010Yves","family":"Winum","sequence":"additional","affiliation":[{"name":"Institut des Biomol\u00e9cules Max Mousseron, IBMM, UMR 5247 CNRS, ENSCM Universit\u00e9 de Montpellier  8 rue de l'Ecole Normale 34296 Montpellier Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,27]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5OB01357G"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201201114"},{"key":"e_1_2_7_3_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201201114"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(19981102)37:20<2754::AID-ANIE2754>3.0.CO;2-3"},{"key":"e_1_2_7_4_3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3757(19981016)110:20<2908::AID-ANGE2908>3.0.CO;2-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","unstructured":"O. 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