{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:15:03Z","timestamp":1783077303551,"version":"3.54.6"},"reference-count":57,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2018,3,5]],"date-time":"2018-03-05T00:00:00Z","timestamp":1520208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2014R1A2A1A11052980"],"award-info":[{"award-number":["2014R1A2A1A11052980"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2018,4,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cysteine plays a crucial role in cellular functions and in human pathologies. However, the development of cysteine probes with extremely accurate detection is still a key challenge for the field. Herein, we have fully characterized and developed a novel selective fluorescent probe: red emission, aqueous detection and large Stokes\u2019 shift for cysteine (<jats:bold>Reals\u2010C<\/jats:bold>). Key in the probe synthesis is a Michael addition onto an acroylate group and subsequent intramolecular cyclization. The probe exhibits analyte detection via an intricate role set up by the leaving groups so to discriminate and form the red\u2010emissive analyte sensing platform (<jats:italic>\u03bb<\/jats:italic><jats:sub>ex<\/jats:sub>=471\u2005nm, <jats:italic>\u03bb<\/jats:italic><jats:sub>em<\/jats:sub>=637\u2005nm) through a chemical cascade pathway. Furthermore, the sensing ability of the probe was demonstrated by both in vitro and in vivo assays. This probe enables for successfully endogenous cysteine sensing in HaCaT human keratinocytes through comparison with a commercial thiol\u2010sensitive probe; <jats:bold>Reals\u2010C<\/jats:bold> shows excellent in vivo cysteine detection in a drug\u2010induced animal liver injury model.<\/jats:p>","DOI":"10.1002\/chem.201706073","type":"journal-article","created":{"date-parts":[[2018,2,5]],"date-time":"2018-02-05T09:59:03Z","timestamp":1517824743000},"page":"5623-5629","source":"Crossref","is-referenced-by-count":35,"title":["Aqueous Red\u2010Emissive Probe for the Selective Fluorescent Detection of Cysteine by Deprotection\/Cyclization Cascade Resulting in Large Stokes\u2019 Shift"],"prefix":"10.1002","volume":"24","author":[{"given":"Youngsam","family":"Kim","sequence":"first","affiliation":[{"name":"Molecular Logic Gate Laboratory Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"},{"name":"Center for Catalytic Hydrocarbon Functionalizations Institute for Basic Science (IBS)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minsuk","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandip V.","family":"Mulay","sequence":"additional","affiliation":[{"name":"Center for Catalytic Hydrocarbon Functionalizations Institute for Basic Science (IBS)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkyung","family":"Jang","sequence":"additional","affiliation":[{"name":"Molecular Logic Gate Laboratory Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Science and Engineering Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo\u2010Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Molecular Logic Gate Laboratory Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6971-586X","authenticated-orcid":false,"given":"Sangyong","family":"Jon","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"},{"name":"Graduate School of Medical Science and Engineering Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2520-4638","authenticated-orcid":false,"given":"David G.","family":"Churchill","sequence":"additional","affiliation":[{"name":"Molecular Logic Gate Laboratory Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"},{"name":"Center for Catalytic Hydrocarbon Functionalizations Institute for Basic Science (IBS)  373-1 Guseong-dong Yuseong-gu Daejeon 305\u2013701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,5]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.6.1636S"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(02)02245-2"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604042200"},{"key":"e_1_2_7_5_1","unstructured":"\u00a0"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2013.02.019"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi001088w"},{"key":"e_1_2_7_8_1","unstructured":"\u00a0"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400645"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1550-2783-2-2-38"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/736837"},{"key":"e_1_2_7_12_1","unstructured":"\u00a0"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2cs35467e"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201604053"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3CS60171D"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr5005524"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201704084"},{"key":"e_1_2_7_17_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201704084"},{"key":"e_1_2_7_18_1","unstructured":"\u00a0"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6CS00192K"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1039\/b910560n"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6AN01942K"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr500567r"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.6b03357"},{"key":"e_1_2_7_24_1","unstructured":"\u00a0"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/b925092a"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3cs60024f"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5CS00152H"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/s121115907"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201103759"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201103759"},{"key":"e_1_2_7_30_1","unstructured":"\u00a0"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2017.07.002"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2sc20540h"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5b10607"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b01384"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.5b00271"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2017.06.166"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.01.019"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA06596D"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochem.2017.06.001"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00377"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/asia.201402043"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA12981D"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3ra47280a"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA06891B"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04329"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2cc33932c"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4TB00190G"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/am500102s"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b04431"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2008.11.007"},{"key":"e_1_2_7_51_1","unstructured":"\u00a0"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3CC46630B"},{"key":"e_1_2_7_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2016.08.020"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.09688"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-00663-0_12"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fchem.201706073","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.201706073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T13:15:23Z","timestamp":1760274923000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.201706073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,5]]},"references-count":57,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2018,4,11]]}},"alternative-id":["10.1002\/chem.201706073"],"URL":"https:\/\/doi.org\/10.1002\/chem.201706073","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,5]]}}}