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LLO\u2013membrane interactions are crucial for pathogenicity of Listeria, but remained unexplained in detail at the molecular level. Here we addressed them by means of <jats:sup>2<\/jats:sup>H, <jats:sup>31<\/jats:sup>P, <jats:sup>13<\/jats:sup>C and <jats:sup>19<\/jats:sup>F solid\u2010state NMR spectroscopy. Different fluid and ordered cholesterol\u2010rich membrane lipid bilayer systems were prepared and checked for the integrity and properties in the presence of LLO. LLO has significantly changed dynamics of phospholipid acyl chains of more fluid cholesterol\u2010rich bilayers, whereas the lipid bilayer organization was not affected. LLO has also affected cholesterol dynamics by increasing the intensity of low frequency motions, indicating direct interactions of LLO with cholesterol. Additionally, the LLO protein was shown to interact differently with lipid membranes, depending on the properties of cholesterol\u2010rich membranes. The presented results, therefore, provide new insights into the interactions of the bacterial toxin LLO with cholesterol\u2010rich membrane systems.<\/jats:p>","DOI":"10.1002\/chem.201802575","type":"journal-article","created":{"date-parts":[[2018,7,6]],"date-time":"2018-07-06T13:15:05Z","timestamp":1530882905000},"page":"14220-14225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Listeriolysin\u2005O Binding Affects Cholesterol and Phospholipid Acyl Chain Dynamics in Fluid Cholesterol\u2010Rich Bilayers"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5379-8517","authenticated-orcid":false,"given":"Mirijam","family":"Kozorog","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Nanobiotechnology National Institute of Chemistry  Hajdrihova 19 1000 Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3284-2176","authenticated-orcid":false,"given":"Marc\u2010Antoine","family":"Sani","sequence":"additional","affiliation":[{"name":"School of Chemistry Bio21 Institute The University of Melbourne  VIC 3010 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6484-2763","authenticated-orcid":false,"given":"Frances","family":"Separovic","sequence":"additional","affiliation":[{"name":"School of Chemistry Bio21 Institute The University of Melbourne  VIC 3010 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9916-8465","authenticated-orcid":false,"given":"Gregor","family":"Anderluh","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Nanobiotechnology National Institute of Chemistry  Hajdrihova 19 1000 Ljubljana Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,28]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"3629","DOI":"10.1128\/iai.57.11.3629-3636.1989","volume":"57","author":"Cossart P.","year":"1989","journal-title":"Infect. 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