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Eu\u2010MDH harbours a redox active 2,7,9\u2010tricarboxypyrroloquinoline quinone (PQQ) cofactor which is non\u2010covalently bound but coordinates trivalent lanthanoid elements including Eu<jats:sup>3+<\/jats:sup>. Eu\u2010MDH and the cytochrome were co\u2010adsorbed with the biopolymer chitosan and cast onto a mercaptoundecanol (MU) monolayer modified Au working electrode. Cyclic voltammetry of cytochrome <jats:italic>c<jats:sub>GJ<\/jats:sub><\/jats:italic> reveals a well\u2010defined quasi\u2010reversible Fe<jats:sup>III\/II<\/jats:sup> redox couple at +255\u2005mV vs. NHE at pH\u20057.5 and this response is pH independent. The reversible one\u2010electron response of the cytochrome <jats:italic>c<jats:sub>GJ<\/jats:sub><\/jats:italic> transforms into a sigmoidal catalytic wave in the presence of Eu\u2010MDH and its substrates (methanol or formaldehyde). The catalytic current was pH\u2010dependent and pH\u20057.3 was found to be optimal. Kinetic parameters (pH dependence, activation energy) obtained by electrochemistry show the same trends as those obtained from an artificial phenazine ethosulfate\/dichlorophenol indophenol assay.<\/jats:p>","DOI":"10.1002\/chem.201900525","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T12:54:22Z","timestamp":1553518462000},"page":"8760-8768","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Electrocatalysis of a Europium\u2010Dependent Bacterial Methanol Dehydrogenase with Its Physiological Electron\u2010Acceptor Cytochrome\u2005<i>c<sub>GJ<\/sub><\/i>"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6335-4012","authenticated-orcid":false,"given":"Palraj","family":"Kalimuthu","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences University of Queensland  Brisbane 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2197-136X","authenticated-orcid":false,"given":"Lena J.","family":"Daumann","sequence":"additional","affiliation":[{"name":"Center for Integrated Protein Science Munich (CIPSM) and Department of Chemistry Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen  Butenandtstr. 5\u201313, Haus D 81377 M\u00fcnchen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Wetland and Water Research Radboud University  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1990-9030","authenticated-orcid":false,"given":"Huub J. 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