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The crude product was a complex mixture consisting mainly of constituent fragments of the O\u2010specific polysaccharide\u2010core (OSPc). The OSPc was only a minor component in the mixture. Two\u2010stage purification of the crude OSPc by HPLC gave pure OSPc fragment of the LPS, as shown by NMR spectroscopy, analytical HPLC and ESI\u2010MS. This material is the purest OSPc fragment of the LPS from\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    O139 reported to date. The purified OSPc was readily converted to the corresponding methyl squarate derivative and the latter was conjugated to BSA. The conjugate, when examined by ELISA, showed immunoreactivity with sera from patients in Bangladesh recovering from cholera caused by\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    O139, but not O1.\n                  <\/jats:p>","DOI":"10.1002\/chem.201902263","type":"journal-article","created":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T13:02:35Z","timestamp":1563195755000},"page":"12946-12956","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Isolation, Purification, Characterization and Direct Conjugation of the Lipid\u2005A\u2010Free Lipopolysaccharide of\n                    <i>Vibrio cholerae<\/i>\n                    O139"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1867-0059","authenticated-orcid":false,"given":"Peng","family":"Xu","sequence":"first","affiliation":[{"name":"Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health  9000 Rockville Bethesda MD 20892-0815 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Korcov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  84238 Bratislava Slovak Republic"},{"name":"Institute of Epidemiology, Faculty of Medicine Comenius University  \u0160pit\u00e1lska 24 81372 Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Bar\u00e1th","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  84238 Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Al\u017ebeta","family":"\u010c\u00ed\u017eov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  84238 Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Val\u00e1rikov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  84238 Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division International Centre for, Diarrhoeal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meagan","family":"Kelly","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Massachusetts General Hospital  Boston MA 02114 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health  9000 Rockville Bethesda MD 20892-0815 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward T.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Massachusetts General Hospital  Boston MA 02114 USA"},{"name":"Harvard Medical School  Boston MA 02115 USA"},{"name":"Department of Immunology and Infectious Diseases Harvard T.H. 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