{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T07:15:45Z","timestamp":1776496545979,"version":"3.51.2"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["H2020-MSCA-ITN-2014-642023"],"award-info":[{"award-number":["H2020-MSCA-ITN-2014-642023"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["15\/CDA\/3648"],"award-info":[{"award-number":["15\/CDA\/3648"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["12\/RC\/2275_P2"],"award-info":[{"award-number":["12\/RC\/2275_P2"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["SFI\/14\/US\/B2997"],"award-info":[{"award-number":["SFI\/14\/US\/B2997"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002808","name":"Carlsbergfondet","doi-asserted-by":"publisher","award":["CF15-0675"],"award-info":[{"award-number":["CF15-0675"]}],"id":[{"id":"10.13039\/501100002808","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["MIS 5002567"],"award-info":[{"award-number":["MIS 5002567"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry A European J"],"published-print":{"date-parts":[[2021,1,13]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report a series of copper(II) artificial metallo\u2010nucleases (AMNs) and demonstrate their DNA damaging properties and <jats:italic>in\u2010vitro<\/jats:italic> cytotoxicity against human\u2010derived pancreatic cancer cells. The compounds combine a tris\u2010chelating polypyridyl ligand, di\u2010(2\u2010pycolyl)amine (DPA), and a DNA intercalating phenanthrene unit. Their general formula is Cu\u2010DPA\u2010<jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>' (where <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>'=1,10\u2010phenanthroline (Phen), dipyridoquinoxaline (DPQ) or dipyridophenazine (DPPZ)). Characterisation was achieved by X\u2010ray crystallography and continuous\u2010wave EPR (cw\u2010EPR), hyperfine sublevel correlation (HYSCORE) and Davies electron\u2010nuclear double resonance (ENDOR) spectroscopies. The presence of the DPA ligand enhances solution stability and facilitates enhanced DNA recognition with apparent binding constants (<jats:italic>K<\/jats:italic><jats:sub>app<\/jats:sub>) rising from 10<jats:sup>5<\/jats:sup> to 10<jats:sup>7<\/jats:sup>\u2009<jats:sc>m<\/jats:sc><jats:sup>\u22121<\/jats:sup> with increasing extent of planar phenanthrene. Cu\u2010DPA\u2010DPPZ, the complex with greatest DNA binding and intercalation effects, recognises the minor groove of guanine\u2013cytosine (G\u2010C) rich sequences. Oxidative DNA damage also occurs in the minor groove and can be inhibited by superoxide and hydroxyl radical trapping agents. The complexes, particularly Cu\u2010DPA\u2010DPPZ, display promising anticancer activity against human pancreatic tumour cells with <jats:italic>in\u2010vitro<\/jats:italic> results surpassing the clinical platinum(II) drug oxaliplatin.<\/jats:p>","DOI":"10.1002\/chem.202001996","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T08:18:38Z","timestamp":1591777118000},"page":"971-983","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Polypyridyl\u2010Based Copper Phenanthrene Complexes: Combining Stability with Enhanced DNA Recognition"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3161-0656","authenticated-orcid":false,"given":"Nicol\u00f3 Zuin","family":"Fantoni","sequence":"first","affiliation":[{"name":"School of Chemical Sciences and National Institute for Cellular, Biotechnology Dublin City University  Glasnevin, Dublin 9 Ireland"},{"name":"Nicol\u00f2 Zuin Fantoni\u2014Chemistry Research Laboratory University of Oxford  12 Mansfield Road Oxford OX1 3TA UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9717-6130","authenticated-orcid":false,"given":"Zara","family":"Molphy","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences and National Institute for Cellular, Biotechnology Dublin City University  Glasnevin, Dublin 9 Ireland"},{"name":"SSPC, the SFI Research Centre for Pharmaceuticals School of Chemical Sciences Dublin City University  Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4901-3542","authenticated-orcid":false,"given":"Sin\u00e9ad","family":"O'Carroll","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences and National Institute for Cellular, Biotechnology Dublin City University  Glasnevin, Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2195-6787","authenticated-orcid":false,"given":"Georgia","family":"Menounou","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerche ISOF  Via P. Gobetti 101 40129 Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7874-2846","authenticated-orcid":false,"given":"George","family":"Mitrikas","sequence":"additional","affiliation":[{"name":"Institute of Nanoscience and Nanotechnology Demokritos NCSR \u201cDemokritos\u201d  Agia Paraskevi Attikis, 153 10 Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8608-6156","authenticated-orcid":false,"given":"Marios G.","family":"Krokidis","sequence":"additional","affiliation":[{"name":"Institute of Nanoscience and Nanotechnology Demokritos NCSR \u201cDemokritos\u201d  Agia Paraskevi Attikis, 153 10 Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-2925","authenticated-orcid":false,"given":"Chryssostomos","family":"Chatgilialoglu","sequence":"additional","affiliation":[{"name":"Consiglio Nazionale delle Ricerche ISOF  Via P. Gobetti 101 40129 Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5694-9010","authenticated-orcid":false,"given":"John","family":"Colleran","sequence":"additional","affiliation":[{"name":"School of Chemical and Pharmaceutical Sciences TU Dublin  Kevin Street Dublin 8 Ireland"},{"name":"Applied Electrochemistry Group Focas Research Institute TU Dublin  Camden Row Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0867-3635","authenticated-orcid":false,"given":"Anna","family":"Banasiak","sequence":"additional","affiliation":[{"name":"Applied Electrochemistry Group Focas Research Institute TU Dublin  Camden Row Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3093-8185","authenticated-orcid":false,"given":"Martin","family":"Clynes","sequence":"additional","affiliation":[{"name":"National Institute for Cellular Biotechnology Dublin City University  Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9697-8876","authenticated-orcid":false,"given":"Sandra","family":"Roche","sequence":"additional","affiliation":[{"name":"National Institute for Cellular Biotechnology Dublin City University  Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suainibhe","family":"Kelly","sequence":"additional","affiliation":[{"name":"National Institute for Cellular Biotechnology Dublin City University  Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7780-5814","authenticated-orcid":false,"given":"Vickie","family":"McKee","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences and National Institute for Cellular, Biotechnology Dublin City University  Glasnevin, Dublin 9 Ireland"},{"name":"Department of Physics, Chemistry and Pharmacy University of Southern Denmark  Campusvej 55 5230 Odense M Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8947-1401","authenticated-orcid":false,"given":"Andrew","family":"Kellett","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences and National Institute for Cellular, Biotechnology Dublin City University  Glasnevin, Dublin 9 Ireland"},{"name":"SSPC, the SFI Research Centre for Pharmaceuticals School of Chemical Sciences Dublin City University  Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,22]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr00022a011"},{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/430_2019_42","volume-title":"The Periodic Table\u2005II: Catalytic, Materials, Biological and Medical Applications","author":"Cardin C. J.","year":"2019"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201814502"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201814502"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5CC04615G"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781788016452-00091"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00157J"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2167"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.11.013"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.5b00597"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9QI00288J"},{"key":"e_1_2_7_11_1","first-page":"319","volume":"37","author":"Hand F.","year":"2019","journal-title":"Surgery"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2017.11.007"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1011923"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8DT00838H"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cc41143e"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.8b00211"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400135x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm-2018-0388"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja026970z"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki856"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr900247m"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86305-6"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201804084"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.8b02526"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03356114"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/00958970008022573"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar950257f"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja012539i"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/B313634E"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic500914j"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/DT9840001349"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0010-8545(00)80135-6"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(74)90298-7"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780198506348.001.0001"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic50204a022"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1670925"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7DT01785E"},{"key":"e_1_2_7_38_1","first-page":"239","volume-title":"Electrochemical Methods: Fundamentals and Applications","author":"Bard A. J.","year":"2001"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60210a007"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cc38710k"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky806"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/362709a0"},{"key":"e_1_2_7_43_1","unstructured":"\u201dSurvival Rates for Pancreatic Cancer \u201d can be found underhttps:\/\/www.cancer.org\/cancer\/pancreatic-cancer\/detection-diagnosis-staging\/survival-rates.html 2020."},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.14740\/wjon1166"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm7010007"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13014-019-1345-6"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21030962"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02630912"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2018.01.008"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2cc30952a"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4DT03010A"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889811043202"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889808042726"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2053273314026370"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2053229614024218"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2005.08.013"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky105"}],"container-title":["Chemistry \u2013 A European Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202001996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/chem.202001996","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/chem.202001996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T09:29:17Z","timestamp":1760261357000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/chem.202001996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,22]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,1,13]]}},"alternative-id":["10.1002\/chem.202001996"],"URL":"https:\/\/doi.org\/10.1002\/chem.202001996","archive":["Portico"],"relation":{},"ISSN":["0947-6539","1521-3765"],"issn-type":[{"value":"0947-6539","type":"print"},{"value":"1521-3765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,22]]},"assertion":[{"value":"2020-04-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}