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To overcome the low solubility of human IAPP, we characterized zinc complexes species formed with 1)\u2005a mutated form of rat\u2010IAPP(1\u201337; R18\u2009H) able to mimic the human IAPP, 2)\u2005the r\u2010IAPP(1\u201337) and the IAPP(1\u20138) fragment. Stoichiometry, speciation and coordination features of zinc(II) complexes were unveiled by ESI\u2010MS, potentiometry and NMR measurements combined with DFT and free\u2010energy simulations. Mononuclear species start to form around pH\u20056; Zn<jats:sup>2+<\/jats:sup> binds both His18 and N\u2010amino terminus in rat\u2010IAPP(1\u201337; R18\u2009H). 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