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However, its biomolecular applications at high magnetic fields (preferably&gt;14\u2005T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl\u2010nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol\u20101, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high\u2010field DNP\/ssNMR spectroscopy, especially for complex biomolecules.<\/jats:p>","DOI":"10.1002\/chem.202102253","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T09:24:12Z","timestamp":1624872252000},"page":"12758-12762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Highly Efficient Trityl\u2010Nitroxide Biradicals for Biomolecular High\u2010Field Dynamic Nuclear Polarization"],"prefix":"10.1002","volume":"27","author":[{"given":"Xinyi.","family":"Cai","sequence":"first","affiliation":[{"name":"The province and ministry co-sponsored collaborative innovation center for medical epigenetics Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics School of Pharmacy Tianjin Medical University  Tianjin 300070 P. 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