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Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine\u2010linked (<jats:italic>N<\/jats:italic>\u2010) glycans in the organelles. In this work, we designed and synthesized an intentionally glycosylated cholera toxin B\u2010subunit (CTB) to be transported to the organelles of mammalian cells. The heptasaccharide, the intermediate structure of various complex\u2010type <jats:italic>N<\/jats:italic>\u2010glycans, was introduced to the CTB. The synthesized monomeric glycosyl\u2010CTB successfully entered mammalian cells and was transported to the Golgi and the ER, suggesting the potential use of synthetic CTB to deliver and investigate the functions of homogeneous <jats:italic>N<\/jats:italic>\u2010glycans in specific organelles of living cells.<\/jats:p>","DOI":"10.1002\/chem.202201253","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T08:40:37Z","timestamp":1653295237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Design and Synthesis of Glycosylated Cholera Toxin B Subunit as a Tracer of Glycoprotein Trafficking in Organelles of Living Cells"],"prefix":"10.1002","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5838-302X","authenticated-orcid":false,"given":"Yuta","family":"Maki","sequence":"first","affiliation":[{"name":"Department of Chemistry Graduate School of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"},{"name":"Project Research Center for Fundamental Sciences Graduate Scholl of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Kawata","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanbo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang\u2010Ying","family":"Goo","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9529-2525","authenticated-orcid":false,"given":"Ryo","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"},{"name":"Project Research Center for Fundamental Sciences Graduate Scholl of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6656-2394","authenticated-orcid":false,"given":"Yasuhiro","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"},{"name":"Project Research Center for Fundamental Sciences Graduate Scholl of Science Osaka University  1-1 Machikaneyama Toyonaka Osaka 560-0043 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3736-1283","authenticated-orcid":false,"given":"Ayano","family":"Satoh","sequence":"additional","affiliation":[{"name":"Graduate School of Interdisciplinary Science and Engineering in Health Systems Okayama University  3-1-1 Tsushimanaka Okayama 700-8530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,23]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u00a0"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5512.2364"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5512.2370"},{"key":"e_1_2_7_4_1","unstructured":"Essentials of Glycobiology (Eds: Varki A. 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