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Interactions between bacterial membrane\u2010bound lectin, Blood group Antigen Binding Adhesin (BabA), and human blood group antigens are key in the initiation of infection. Herein, the synthesis of a B\u2010antigen hexasaccharide (B6) and a B\u2010Lewis b heptasaccharide (BLeb7) and Bovine Serum Albumin glycoconjugates thereof is reported to assess the binding properties and preferences of BabA from different strains. From a previously reported trisaccharide acceptor a versatile key Lacto\u2010<jats:italic>N<\/jats:italic>\u2010tetraose tetrasaccharide intermediate was synthesized, which allowed us to explore various routes to the final targets, either via initial introduction of fucosyl residues followed by introduction of the B\u2010determinant or vice versa. The first approach proved unsuccessful, whereas the second afforded the target structures in good yields. Protein conjugation using isothiocyanate methodology allowed us to reach high glycan loadings (up to 23 per protein) to mimic multivalent displays encountered in Nature. Protein glycoconjugate inhibition binding studies were performed with <jats:italic>H. pylori<\/jats:italic> strains displaying high or low affinity for Lewis b hexasaccharide structures showing that the binding to the high affinity strain was reduced due to the presence of the B\u2010determinant in the Bleb7\u2010conjugates and further reduced by the absence of the Lewis fucose residue in the B6\u2010conjugate.<\/jats:p>","DOI":"10.1002\/chem.202203672","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T04:54:46Z","timestamp":1671771286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Synthesis of a B\u2010Antigen Hexasaccharide, a B\u2010Lewis b Heptasaccharide and Glycoconjugates Thereof to Investigate Binding Properties of <i>Helicobacter pylori<\/i>"],"prefix":"10.1002","volume":"29","author":[{"given":"Mark","family":"Reihill","sequence":"first","affiliation":[{"name":"Centre for Synthesis and Chemical Biology University College Dublin  Belfield Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane","family":"Fourni\u00e8re","sequence":"additional","affiliation":[{"name":"School of Natural Sciences Bangor University  Bangor Gwynedd LL57 2UW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisling N\u00ed","family":"Cheallaigh","sequence":"additional","affiliation":[{"name":"Centre for Synthesis and Chemical Biology University College Dublin  Belfield Dublin 4 Ireland"},{"name":"Lennard-Jones Laboratories Centre for Glycoscience Research Keele University  Staffordshire ST5 5BG UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan Olofsson","family":"Edlund","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics Ume\u00e5 University  90187 Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin John","family":"Miller","sequence":"additional","affiliation":[{"name":"Lennard-Jones Laboratories Centre for Glycoscience Research Keele University  Staffordshire ST5 5BG UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bor\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Biophysics Ume\u00e5 University  90187 Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Lahmann","sequence":"additional","affiliation":[{"name":"School of Natural Sciences Bangor University  Bangor Gwynedd LL57 2UW UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8273-4918","authenticated-orcid":false,"given":"Stefan","family":"Oscarson","sequence":"additional","affiliation":[{"name":"Centre for Synthesis and 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