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The radiopharmacological characterization of the two isomeric bispidine complexes includes quantitative labeling with <jats:sup>64<\/jats:sup>Cu<jats:sup>II<\/jats:sup> at ambient conditions with high radiochemical purities and yields (molar activities &gt;200\u2005MBq\/nmol). Challenge experiments in presence of EDTA, cyclam, human serum and SOD demonstrate high stability and inertness of the <jats:sup>64<\/jats:sup>Cu\u2010bispidine complexes. Biodistribution studies performed in Wistar rats indicate a rapid renal elimination for both <jats:sup>64<\/jats:sup>Cu\u2010labeled chelates. The bispidine ligand with the picolinate group in N7 position was selected for further biological experiments, and its backbone was therefore substituted with a benzyl\u2010NCS group at C9. Two tumor target modules (TMs), targeting prostate stem cell antigen (PSCA), overexpressed in prostate cancer, and the fibroblast activation protein (FAP) in fibrosarcoma, were selected for thiourea coupling with the NCS\u2010functionalized ligand and lysine residues of TMs. Small animal PET experiments on tumor\u2010bearing mice showed specific accumulation of the <jats:sup>64<\/jats:sup>Cu\u2010labeled TMs in PSCA\u2010 and FAP\u2010overexpressing tumors (standardized uptake value (SUV) for PC3: 2.7\u00b10.6 and HT1080: 7.2\u00b11.25) with almost no uptake in wild type tumors.<\/jats:p>","DOI":"10.1002\/chem.202400366","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T06:39:13Z","timestamp":1710916753000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<sup>64<\/sup>Cu tumor labeling with hexadentate picolinic acid\u2010based bispidine 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