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However, synthetic oligosaccharide antigens have become a promising alternative to natural polysaccharides with the advantage of being well\u2010defined structures providing homogeneous conjugates.\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    (Hi) is responsible for a number of severe diseases. In recent years, an increasing rate of invasive infections caused by Hi serotype a (Hia) raised some concern, because no vaccine targeting Hia is currently available. The capsular polysaccharide (CPS) of Hia is constituted by phosphodiester\u2010linked 4\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glucose\u2010(1\u21924)\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010ribitol\u20105\u2010(PO\n                    <jats:sub>4<\/jats:sub>\n                    \u2192) repeating units and is the antigen for protein\u2010conjugated polysaccharide vaccines. To investigate the antigenic potential of the CPS from Hia, we synthesized related saccharide fragments containing up to five repeating units. Following the synthetic optimization of the needed disaccharide building blocks, they were assembled using the phosphoramidite approach for the installation of the phosphodiester linkages. The resulting CPS\u2010based Hia oligomers were conjugated to CRM\n                    <jats:sub>197<\/jats:sub>\n                    carrier protein and evaluated in\u2005vivo for their immunogenic potential, showing that all glycoconjugates were capable of raising antibodies recognizing Hia synthetic fragments.\n                  <\/jats:p>","DOI":"10.1002\/chem.202401695","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T14:04:10Z","timestamp":1718719450000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Semisynthetic Glycoconjugates as Potential Vaccine Candidates Against\n                    <i>Haemophilus influenzae<\/i>\n                    Type a"],"prefix":"10.1002","volume":"30","author":[{"given":"Claudia V.","family":"Kohout","sequence":"first","affiliation":[{"name":"Department of Chemistry Universit\u00e0 degli Studi di Milano  Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Del Bino","sequence":"additional","affiliation":[{"name":"GSK, Research Centre  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Petrosilli","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e0 degli Studi di Milano  Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"D'Orazio","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e0 degli Studi di Milano  Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria R.","family":"Romano","sequence":"additional","affiliation":[{"name":"GSK, Research Centre  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen D. 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